Cannabidiol inhibits human glioma cell migration through a cannabinoid receptor-independent mechanism

被引：131

作者：

Vaccani, A

Massi, P

Colombo, A

Rubino, T

Parolaro, D

机构：

[1] Univ Insubria, Ctr Neurosci, Dept Struct & Funct Biol, Pharmacol Sect, I-21052 Busto Arsizio, VA, Italy

[2] Univ Milan, Dept Pharmacol Chemotherapy & Med Toxicol, I-20129 Milan, Italy

来源：

BRITISH JOURNAL OF PHARMACOLOGY | 2005年 / 144卷 / 08期

关键词：

cannabidiol; glioma cells; cell migration;

D O I：

10.1038/sj.bjp.0706134

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

We evaluated the ability of cannabidiol (CBD) to impair the migration of tumor cells stimulated by conditioned medium. CBD caused concentration-dependent inhibition of the migration of U87 glioma cells, quantified in a Boyden chamber. Since these cells express both cannabinoid CB1 and CB2 receptors in the membrane, we also evaluated their engagement in the antimigratory effect of CBD. The inhibition of cell was not antagonized either by the selective cannabinoid receptor antagonists SR141716 ( CB1) and SR144528 ( CB2) or by pretreatment with pertussis toxin, indicating no involvement of classical cannabinoid receptors and/or receptors coupled to Gi/o proteins. These results reinforce the evidence of antitumoral properties of CBD, demonstrating its ability to limit tumor invasion, although the mechanism of its pharmacological effects remains to be clarified.

引用

收藏

页码：1032 / 1036

页数：5

相关论文

共 17 条

[1] Molecular targets for cannabidiol and its synthetic analogues: effect on vanilloid VR1 receptors and on the cellular uptake and enzymatic hydrolysis of anandamide [J].

Bisogno, T ;

Hanus, L ;

De Petrocellis, L ;

Tchilibon, S ;

Ponde, DE ;

Brandi, I ;

Moriello, AS ;

Davis, JB ;

Mechoulam, R ;

Di Marzo, V .

BRITISH JOURNAL OF PHARMACOLOGY, 2001, 134 (04) :845-852

[2] Differential expression of the CB2 cannabinoid receptor by rodent macrophages and macrophage-like cells in relation to cell activation [J].

Carlisle, SJ ;

Marciano-Cabral, F ;

Staab, A ;

Ludwick, C ;

Cabral, GA .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2002, 2 (01) :69-82

[3] Cannabinoids:: Potential anticancer agents [J].

Guzmán, M .

NATURE REVIEWS CANCER, 2003, 3 (10) :745-755

[4] International Union of Pharmacology. XXVII. Classification of cannabinoid receptors [J].

Howlett, AC ;

Barth, F ;

Bonner, TI ;

Cabral, G ;

Casellas, P ;

Devane, WA ;

Felder, CC ;

Herkenham, M ;

Mackie, K ;

Martin, BR ;

Mechoulam, R ;

Pertwee, RG .

PHARMACOLOGICAL REVIEWS, 2002, 54 (02) :161-202

[5] Cannabinoid-induced mesenteric vasodilation through an endothelial site distinct from CB1 or CB2 receptors [J].

Járai, Z ;

Wagner, JA ;

Varga, K ;

Lake, KD ;

Compton, DR ;

Martin, BR ;

Zimmer, AM ;

Bonner, TI ;

Buckley, NE ;

Mezey, E ;

Razdan, RK ;

Zimmer, A ;

Kunos, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (24) :14136-14141

[6]

Jordà MA, 2002, BLOOD, V99, P2786

[7] Anandamide is an endogenous inhibitor for the migration of tumor cells and T lymphocytes [J].

Joseph, J ;

Niggemann, B ;

Zaenker, KS ;

Entschladen, F .

CANCER IMMUNOLOGY IMMUNOTHERAPY, 2004, 53 (08) :723-728

[8] 2-Arachidonoylglycerol induces the migration of HL-60 cells differentiated into macrophage-like cells and human peripheral blood monocytes through the cannabinoid CB2 receptor-dependent mechanism [J].

Kishimoto, S ;

Gokoh, M ;

Oka, S ;

Muramatsu, M ;

Kajiwara, T ;

Waku, K ;

Sugiura, T .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (27) :24469-24475

[9] Antitumor effects of cannabidiol, a nonpsychoactive cannabinoid, on human glioma cell lines [J].

Massi, P ;

Vaccani, A ;

Ceruti, S ;

Colombo, A ;

Abbracchio, MP ;

Parolaro, D .

JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2004, 308 (03) :838-845

[10] Endocannabinoids in the immune system and cancer [J].

Parolaro, D ;

Massi, P ;

Rubino, T ;

Monti, E .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 2002, 66 (2-3) :319-332