Peroxynitrite cytotoxicity on bovine retinal pigmented epithelial cells in culture

被引：56

作者：

BeharCohen, FF ^{[1
]}

Heydolph, S ^{[1
]}

Faure, V ^{[1
]}

DroyLefaix, MT ^{[1
]}

Courtois, Y ^{[1
]}

Goureau, O ^{[1
]}

机构：

[1] IPSEN INST,F-75016 PARIS,FRANCE

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 226卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.1438

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peroxynitrite induced in vitro a dose dependent toxicity on retinal pigmented epithelial (RPE) cells. Cell death was partially mediated by apoptosis as demonstrated by nuclear fragmentation and TdT-mediated dUTP nick-end labeling assay. Peroxynitrite-induced tyrosine nitration was revealed by immunocytochemistry, both in the cytoplasm and in the nucleus of the cells. Nitration was not observed in RPE cells, producing nitric oxide (NO) after stimulation by lipopolysacharide and interferon-g (IFN-gamma), suggesting that peroxynitrite was not formed in vitro in such conditions. Peroxynitrite could be responsible for the retinal damages observed in pathological conditions in which NO has been demonstrated to be involved. In this context, EGb761, identified as a free radical scavenger, was showed herein to protect RPE cells against peroxynitrite injury. (C) 1996 Academic Press, Inc.

引用

页码：842 / 849

页数：8

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[1] Activation of the inducible form of nitric oxide synthase in the brains of patients with multiple sclerosis [J].