Evaluation of Swabs, Transport Media, and Specimen Transport Conditions for Optimal Detection of Viruses by PCR

被引：85

作者：

Druce, Julian ^{[1
]}

Garcia, Katherine ^{[1
]}

Tran, Thomas ^{[1
]}

Papadakis, Georgina ^{[1
]}

Birch, Chris ^{[1
]}

机构：

[1] Victorian Infect Dis Reference Lab, Melbourne, Vic, Australia

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2012年 / 50卷 / 03期

关键词：

RESPIRATORY VIRUSES; FLOCKED SWABS; COLLECTION; SURVIVAL; SURFACES;

D O I：

10.1128/JCM.06551-11

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Depletion of swabs and viral transport medium during epidemics may prompt the use of unvalidated alternatives. Swabs collected and transported dry or in saline were compared to commercially available swab/medium combinations for PCR detection of influenza, enterovirus, herpes simplex virus, and adenovirus. Each was detected at an ambient temperature (22 degrees C) and 4 degrees C for 7 days. Detection of influenza on dry or saline swabs is important because of its capacity to cause outbreaks involving large numbers of cases.

引用

页码：1064 / 1065

页数：2

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