Development and application of a high resolution liquid chromatographic method for the analysis of complex pigment distributions

被引：124

作者：

Airs, RL ^{[1
]}

Atkinson, JE ^{[1
]}

Keely, BJ ^{[1
]}

机构：

[1] Univ York, Dept Chem, York YO10 5DD, N Yorkshire, England

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 917卷 / 1-2期

基金：

英国工程与自然科学研究理事会;

关键词：

Chlorobium phaeobacteroides; pigments;

D O I：

10.1016/S0021-9673(01)00663-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ternary and binary gradient systems have been developed for the high-performance liquid chromatographic analysis of complex pigment distributions typical of natural samples. Improved chromatographic resolution reveals significantly more pigment components in extracts from a sediment (Priest Pot, Cumbria, UK), a microbial mat (les Salines de la Trinital, South Catalonia, Spain) and a culture (C. phaeobacteroides) including novel bacteriochlorophyll derivatives. The methods developed are directly suited to LC-MS analysis and the automated acquisition of MS/MS data for pigments. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：167 / 177

页数：11

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