Trifluoromethanesulfonic acid as a catalyst for the formation of dansylhydrazone derivatives

被引:14
作者
Hyytiainen, M
Appelblad, P
Ponten, E
Stigbrand, M
Irgum, K
Jaegfeldt, H
机构
[1] UMEA UNIV,DEPT ANALYT CHEM,S-90187 UMEA,SWEDEN
[2] ASTRO DRACO AB,BIOANALYT CHEM,S-22100 LUND,SWEDEN
关键词
derivatization; LC; trifluoromethanesulfonic acid; steroids; budesonide; dansylhydrazones;
D O I
10.1016/0021-9673(96)00256-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Trifluoromethanesulfonic acid (TFMSA) is presented as a new, efficient catalyst in the pre-column fluorescent derivatization of the 3-ketosteroid budesonide with dansylhydrazine and compared to the commonly used catalyst trifluoroacetic acid. With TFMSA the derivatization reaction may be carried out at room temperature, with a considerably higher reaction rate compared to previously used acids. The chromatograms also show that TFMSA results in less formation of spurious peaks from the reagent. Derivatization of steroid standard solutions ranging from 0.5 to 64 mu M could be performed using identical reaction conditions.
引用
收藏
页码:279 / 283
页数:5
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