Fatty acids modulate lecithin:cholesterol acyltransferase secretion independently of effects on triglyceride secretion in primary rat hepatocytes

被引:16
作者
Fungwe, TV [1 ]
Kudchodkar, BJ
Lacko, AG
Dory, L
机构
[1] Wayne State Univ, Dept Nutr & Food Sci, Detroit, MI 48202 USA
[2] Univ N Texas, Hlth Sci Ctr, Dept Biochem & Mol Biol, Ft Worth, TX 76107 USA
关键词
lecithin : cholesterol acyltransferase; fatty acids; secretion; gene expression; rat hepatocytes;
D O I
10.1093/jn/128.8.1270
中图分类号
R15 [营养卫生、食品卫生]; TS201 [基础科学];
学科分类号
100403 ;
摘要
The regulation of plasma lecithin:cholesterol acyltransferase (LCAT) expression is not well understood. Although oleic acid increases both the secretion of triglycerides and LCAT by primary rat hepatocytes, the effect of other fatty acids (FA) on LCAT secretion is not known. This study was designed to examine the effect of FA on the hepatic secretion of LCAT, triglyceride and apolipoprotein A-1 (apoA-1), Primary rat hepatocytes were incubated with serum-free medium, supplemented with individual FA (0-1 mmol/L) for 22-24 h, Preliminary studies indicated a linear secretion of LCAT up to 24 h in both control and FA-treated cells. When hepatocytes were incubated with 1 mmol/L FA, the LCAT secretion increased 50-100% (P < 0.01) in the presence of the 18-carbon FA (stearic, oleic, elaidic and linoleic acids), whereas the presence of butyric, lauric and palmitic acids had no significant effect. LCAT secretion decreased (P < 0.01) in the presence of docosahexaenoic acid (DHA). All FA (except DHA) significantly enhanced triglyceride secretion; however, only the 18 carbon FA significantly stimulated the synthesis and secretion of apoA-1 and secretion of LCAT, The secretion of LCAT correlated with apoA-1 secretion (r = 0.88, P = 0.004) but not with triglyceride secretion (r = 0.55, P = 0.12). Treatment with oleic acid resulted in a 1.5-fold increase in hepatocyte LCAT mRNA accumulation, whereas butyrate and palmitate had no effect. These data indicate that FA that promote the apparent synthesis and secretion of apoA-1 also stimulate the secretion of LCAT in vitro, suggesting a coordinate regulatory mechanism for apoA-1 and LCAT expression.
引用
收藏
页码:1270 / 1275
页数:6
相关论文
共 38 条
[1]   POSITIVE CORRELATION OF SERUM LECITHIN - CHOLESTEROL ACYLTRANSFERASE ACTIVITY WITH RELATIVE BODY-WEIGHT [J].
AKANUMA, Y ;
KUZUYA, T ;
HAYASHI, M ;
IDE, T ;
KUZUYA, N .
EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 1973, 3 (02) :136-141
[2]  
ALBERS JJ, 1981, J LIPID RES, V22, P1206
[3]  
Assmann G., 1991, CURR OPIN LIPIDOL, V2, P110
[4]   DIETARY-FAT ELEVATES HEPATIC APOA-I PRODUCTION BY INCREASING THE FRACTION OF APOLIPOPROTEIN-A-I MESSENGER-RNA IN THE TRANSLATING POOL [J].
AZROLAN, N ;
ODAKA, H ;
BRESLOW, JL ;
FISHER, EA .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (34) :19833-19838
[5]   SINGLE BILAYER LIPOSOMES PREPARED WITHOUT SONICATION [J].
BATZRI, S ;
KORN, ED .
BIOCHIMICA ET BIOPHYSICA ACTA, 1973, 298 (04) :1015-1019
[6]  
Berry M.N., 1991, Isolated hepatocytes. Properties, V1st edition
[7]   CHARACTERIZATION OF HUMAN HIGH-DENSITY LIPOPROTEINS BY GRADIENT GEL-ELECTROPHORESIS [J].
BLANCHE, PJ ;
GONG, EL ;
FORTE, TM ;
NICHOLS, AV .
BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 665 (03) :408-419
[8]   A LOW-FAT DIET DECREASES HIGH-DENSITY LIPOPROTEIN (HDL) CHOLESTEROL LEVELS BY DECREASING HDL APOLIPOPROTEIN TRANSPORT RATES [J].
BRINTON, EA ;
EISENBERG, S ;
BRESLOW, JL .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 85 (01) :144-151
[9]   HUMAN HDL CHOLESTEROL LEVELS ARE DETERMINED BY APOA-I FRACTIONAL CATABOLIC RATE, WHICH CORRELATES INVERSELY WITH ESTIMATES OF HDL PARTICLE-SIZE [J].
BRINTON, EA ;
EISENBERG, S ;
BRESLOW, JL .
ARTERIOSCLEROSIS AND THROMBOSIS, 1994, 14 (05) :707-720
[10]  
CHEUNG MC, 1986, J LIPID RES, V27, P1135