Survival of Campylobacter jejuni strains from different origins under oxidative stress conditions:: Effect of temperature

被引：55

作者：

Garenaux, A. ^{[1
]}

Jugiau, F. ^{[1
]}

Rama, Florence ^{[1
]}

de Jonge, R. ^{[2
]}

Denis, M. ^{[3
]}

Federighi, M. ^{[1
]}

Ritz, M. ^{[1
]}

机构：

[1] Ecole Natl Vet Nantes, INRA, UMR 1014, SECALIM ENVN ENITIAA, F-44307 Nantes 03, France

[2] Natl Inst Publ Hlth & Environm, Microbiol Lab Publ Hlth, NL-3720 BA Bilthoven, Netherlands

[3] Agcy Francaise Securite Sanit Aliments, Unite HQPAP, F-22440 Ploufragan, France

来源：

CURRENT MICROBIOLOGY | 2008年 / 56卷 / 04期

关键词：

D O I：

10.1007/s00284-007-9082-8

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Campylobacter jejuni is a microaerophilic pathogen but is able to survive oxidative stress conditions during its transmission to the human host. Strains of different origins (reference, poultry, or human clinical) were tested for survival under oxidative stress conditions. C. jejuni strains were grown in Mueller Hinton broth to obtain late exponential-phase cultures. Then they were exposed to 2 different stresses: (1) cultures were either plated on Columbia agar plates and exposed to atmospheric oxygen or (2) paraquat (a chemical oxidizing agent) was added to liquid cultures to reach a 500-mu M concentration. Both of these experimental conditions were realized at 3 different temperatures: 4 degrees C, 25 degrees C, and 42 degrees C. Results obtained with paraquat and atmospheric oxygen were similar. Surprisingly, C. jejuni was found to be very sensitive to oxidative stress at 42 degrees C, which is its optimal growth temperature, whereas it was more resistant at 4 degrees C. A strain effect was observed, but no relationship was found between the origin of the strains and level of resistance. High temperature (42 degrees C) combined with oxidative stress allowed a rapid decrease in the C. jejuni population, whereas low temperature considerably decreased the effect of oxidative stress.

引用

页码：293 / 297

页数：5

共 20 条

[1]

Allos BM, 2001, CLIN INFECT DIS, V32, P1201, DOI 10.1086/319760

[2] Aerobic growth and survival of Campylobacter jejuni in food and stream water [J].

Chynoweth, RW ;

Hudson, JA ;

Thom, K .

LETTERS IN APPLIED MICROBIOLOGY, 1998, 27 (06) :341-344

[3] Comparison of virulence-associated in vitro properties of typed strains of Campylobacter jejuni from different sources [J].

Coote, J. G. ;

Stewart-Tull, D. E. S. ;

Owen, R. J. ;

Bolton, F. J. ;

Siemer, Berit L. ;

Candlish, Denise ;

Thompson, D. H. ;

Wardlaw, A. C. ;

On, S. L. W. ;

Candlish, A. ;

Billcliffe, Bronwen ;

Jordan, Penelope J. ;

Kristiansen, K. ;

Borman, Pauline .

JOURNAL OF MEDICAL MICROBIOLOGY, 2007, 56 (06) :722-732

[4]

*EUR FOOD SAF AUTH, 2005, EFSA J, P94

[5] OXIDATIVE STRESS RESPONSES IN ESCHERICHIA-COLI AND SALMONELLA-TYPHIMURIUM [J].

FARR, SB ;

KOGOMA, T .

MICROBIOLOGICAL REVIEWS, 1991, 55 (04) :561-585

[6] Impact of different storage factors on the survivability of Campylobacter jejuni in turkey meat [J].

Haenel, Christoph M. ;

Atanassova, Viktoria .

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2007, 49 (01) :146-148

[7]

Hazeleger WC, 1998, APPL ENVIRON MICROB, V64, P3917

[8] Unique features of a highly pathogenic Campylobacter jejuni strain [J].

Hofreuter, Dirk ;

Tsai, Jennifer ;

Watson, Robert O. ;

Novik, Veronica ;

Altman, Bill ;

Benitez, Michelle ;

Clark, Christina ;

Perbost, Clotilde ;

Jarvie, Thomas ;

Du, Lei ;

Galan, Jorge E. .

INFECTION AND IMMUNITY, 2006, 74 (08) :4694-4707

[9] The Campylobacter conundrum [J].

Jones, K .

TRENDS IN MICROBIOLOGY, 2001, 9 (08) :365-366

[10] ADHESION TO AND INVASION OF HEP-2 CELLS BY CAMPYLOBACTER SPP [J].

KONKEL, ME ;

JOENS, LA .

INFECTION AND IMMUNITY, 1989, 57 (10) :2984-2990

← 1 2 →