The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress

被引:67
作者
Kocourkova, Daniela [1 ]
Krckova, Zuzana [1 ]
Pejchar, Premysl [1 ]
Veselkova, Stepanka [1 ]
Valentova, Olga [2 ]
Wimalasekera, Rinukshi [3 ]
Scherer, Guenther F. E. [3 ]
Martinec, Jan [1 ]
机构
[1] Acad Sci Czech Republ, Inst Expt Bot, Vvi, CR-16502 Prague 6, Czech Republic
[2] Prague Inst Chem Technol, Dept Biochem & Microbiol, Fac Food & Biochem Technol, CR-16628 Prague 6, Czech Republic
[3] Leibniz Univ Hannover, Sect Appl Mol Physiol, Inst Floriculture & Wood Sci, D-30419 Hannover, Germany
关键词
Arabidopsis thaliana; diacylglycerol; phospholipase C; salt stress; PHOSPHATIDIC-ACID; PLASMA-MEMBRANE; SIGNAL-TRANSDUCTION; DROUGHT TOLERANCE; GENE-EXPRESSION; THALIANA; ABI1; FAMILY; RAB18; SOS1;
D O I
10.1093/jxb/err039
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Phosphatidylcholine-hydrolysing phospholipase C, also known as non-specific phospholipase C (NPC), is a new member of the plant phospholipase family that reacts to environmental stresses such as phosphate deficiency and aluminium toxicity, and has a role in root development and brassinolide signalling. Expression of NPC4, one of the six NPC genes in Arabidopsis, was highly induced by NaCl. Maximum expression was observed from 3 h to 6 h after the salt treatment and was dependent on salt concentration. Results of histochemical analysis of P-NPC4:GUS plants showed the localization of salt-induced expression in root tips. On the biochemical level, increased NPC enzyme activity, indicated by accumulation of diacylglycerol, was observed as early as after 30 min of salt treatment of Arabidopsis seedlings. Phenotype analysis of NPC4 knockout plants showed increased sensitivity to salinity as compared with wild-type plants. Under salt stress npc4 plants had shorter roots, lower fresh weight, and reduced seed germination. Expression levels of abscisic acid-related genes ABI1, ABI2, RAB18, PP2CA, and SOT12 were substantially reduced in salt-treated npc4 plants. These observations demonstrate a role for NPC4 in the response of Arabidopsis to salt stress.
引用
收藏
页码:3753 / 3763
页数:11
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