Robust regression methods for real-time polymerase chain reaction

被引：7

作者：

Trypsteen, Wim ^{[1
,2
]}

De Neve, Jan ^{[3
]}

Bosman, Kobus ^{[4
]}

Nijhuis, Monique ^{[4
]}

Thas, Olivier ^{[3
,5
]}

Vandekerckhove, Linos ^{[1
,2
]}

De Spiegelaere, Ward ^{[1
,2
]}

机构：

[1] Univ Ghent, Dept Internal Med, HIV Translat Res Unit, B-9000 Ghent, Belgium

[2] Ghent Univ Hosp, B-9000 Ghent, Belgium

[3] Univ Ghent, Dept Math Modelling Stat & Bioinformat, B-9000 Ghent, Belgium

[4] Univ Med Ctr Utrecht, Dept Med Microbiol & Virol, NL-3584 CX Utrecht, Netherlands

[5] Univ Wollongong, Sch Math & Appl Stat, NIASRA, Wollongong, NSW 2522, Australia

来源：

ANALYTICAL BIOCHEMISTRY | 2015年 / 480卷

关键词：

Robust regression; Real-time PCR; Outliers; qPCR; Standard curve; PCR efficiency estimation;

D O I：

10.1016/j.ab.2015.04.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Current real-time polymerase chain reaction (PCR) data analysis methods implement linear least squares regression methods for primer efficiency estimation based on standard curve dilution series. This method is sensitive to outliers that distort the outcome and are often ignored or removed by the end user. Here, robust regression methods are shown to provide a reliable alternative because they are less affected by outliers and often result in more precise primer efficiency estimators than the linear least squares method. (C) 2015 Elsevier Inc. All rights reserved.

引用

页码：34 / 36

页数：3

共 15 条

[1] [Anonymous], 2003, Robust Regression and Outlier Detection
[2] [Anonymous], 1950, InNed. Akad. Wetensch. Proc. Ser. A
[3] [Anonymous], BMC BIOTECHNOL
[4] The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Bustin, Stephen A.
Benes, Vladimir
Garson, Jeremy A.
Hellemans, Jan
Huggett, Jim
Kubista, Mikael
Mueller, Reinhold
Nolan, Tania
Pfaffl, Michael W.
Shipley, Gregory L.
Vandesompele, Jo
Wittwer, Carl T.
[J]. CLINICAL CHEMISTRY, 2009, 55 (04) : 611 - 622
[5] FOLKS TM, 1988, J IMMUNOL, V140, P1117
[6] Real time quantitative PCR
Heid, CA
Stevens, J
Livak, KJ
Williams, PM
[J]. GENOME RESEARCH, 1996, 6 (10): : 986 - 994
[7] RNA-directed gene editing specifically eradicates latent and prevents new HIV-1 infection
Hu, Wenhui
Kaminski, Rafal
Yang, Fan
Zhang, Yonggang
Cosentino, Laura
Li, Fang
Luo, Biao
Alvarez-Carbonell, David
Garcia-Mesa, Yoelvis
Karn, Jonathan
Mo, Xianming
Khalili, Kamel
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (31) : 11461 - 11466
[8] Performance of robust regression methods in real-time polymerase chain reaction calibration
Orenti, Annalisa
Marubini, Ettore
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MARKERS, 2014, 29 (04) : E317 - E327
[9] R Core Team, 2014, R LANG ENV STAT COMP
[10] Evaluation of qPCR curve analysis methods for reliable biomarker discovery: Bias, resolution, precision, and implications
Ruijter, Jan M.
Pfaffl, Michael W.
Zhao, Sheng
Spiess, Andrej N.
Boggy, Gregory
Blom, Jochen
Rutledge, Robert G.
Sisti, Davide
Lievens, Antoon
De Preter, Katleen
Derveaux, Stefaan
Hellemans, Jan
Vandesompele, Jo
[J]. METHODS, 2013, 59 (01) : 32 - 46

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