Antioxidant activity of the phenol rich fractions of leaves of Chukrasia tabularis A.!Juss

被引：74

作者：

Kaur, Rajbir ^{[1
]}

Arora, Saroi ^{[1
]}

Singh, Bikram ^{[2
]}

机构：

[1] Guru Nanak Dev Univ, Dept Bot & Environm Sci, Amritsar 143005, Punjab, India

[2] IHBT, Nat Plant Prod Div, Palampur 176061, India

来源：

BIORESOURCE TECHNOLOGY | 2008年 / 99卷 / 16期

关键词：

Chukrasia tabularis; phenolic compounds; free radical scavenging activities; DNA nicking assay; total phenol content;

D O I：

10.1016/j.biortech.2008.01.070

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The present study was designed to explore the antioxidant potential of chloroform, ethyl acetate, n-butanol and water fractions of 80% methanol extract of leaves Chukrasia tabularis by 2,2'-diphenyl- I -picrylhydrazyl (DPPH), deoxyribose degradation (non-site specific and site specific), reducing power and DNA nicking assays. The different fractions showed significant activities in all the free radical scavenging tests and these findings have also shown direct relationship between antioxidant activity and phenolic content. Among the fractions, ethyl acetate fraction exhibited highest inhibition of 93.14%, 89.99%, 87.04% in DPPH, non-site specific and site specific deoxyribose degradation assays, respectively and 91.20% reduction of ferricyanide to give Prussian blue coloured complex in reducing power assay at maximum concentration tested. This preliminary study indicates the antioxidant activity of the leaves of Chukrasia tabularis, and moreover the results showed correlation with the amount of phenolic content present in different fractions. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：7692 / 7698

页数：7

共 29 条

[1] Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies [J].

Amarowicz, R ;

Pegg, RB ;

Rahimi-Moghaddam, P ;

Barl, B ;

Weil, JA .

FOOD CHEMISTRY, 2004, 84 (04) :551-562

[2]

AROUMA OI, 1987, J INORG CHEM, V29, P289

[3]

Bazzano LA, 2002, AM J CLIN NUTR, V76, P93

[4] FRUIT, VEGETABLES, AND CANCER PREVENTION - A REVIEW OF THE EPIDEMIOLOGIC EVIDENCE [J].

BLOCK, G ;

PATTERSON, B ;

SUBAR, A .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 1992, 18 (01) :1-29

[5] ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].

BLOIS, MS .

NATURE, 1958, 181 (4617) :1199-1200

[6] Antioxidant and prooxidant behavior of flavonoids: Structure-activity relationships [J].

Cao, GH ;

Sofic, E ;

Prior, RL .

FREE RADICAL BIOLOGY AND MEDICINE, 1997, 22 (05) :749-760

[7] Tabularisins A-D, phragmalin ortho esters with new skeleton isolated from the seeds of Chukrasia tabularis [J].

Fan, Cheng-Qi ;

Wang, Xiao-Ning ;

Yin, Sheng ;

Zhang, Chuan-Rui ;

Wang, Fang-Dao ;

Yue, Jian-Min .

TETRAHEDRON, 2007, 63 (29) :6741-6747

[8] FERROUS-SALT-PROMOTED DAMAGE TO DEOXYRIBOSE AND BENZOATE - THE INCREASED EFFECTIVENESS OF HYDROXYL-RADICAL SCAVANGERS IN THE PRESENCE OF EDTA [J].

GUTTERIDGE, JMC .

BIOCHEMICAL JOURNAL, 1987, 243 (03) :709-714

[9] THE DEOXYRIBOSE METHOD - A SIMPLE TEST-TUBE ASSAY FOR DETERMINATION OF RATE CONSTANTS FOR REACTIONS OF HYDROXYL RADICALS [J].

HALLIWELL, B ;

GUTTERIDGE, JMC ;

ARUOMA, OI .

ANALYTICAL BIOCHEMISTRY, 1987, 165 (01) :215-219

[10] The chemistry behind antioxidant capacity assays [J].

Huang, DJ ;

Ou, BX ;

Prior, RL .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2005, 53 (06) :1841-1856

← 1 2 3 →