Simultaneous quantitation of ephedrines in urine by gas chromatography-nitrogen-phosphorus detection for doping control purposes

被引：51

作者：

Van Eenoo, P ^{[1
]}

Delbeke, FT ^{[1
]}

Roels, K ^{[1
]}

De Backer, P ^{[1
]}

机构：

[1] State Univ Ghent, Doping Control Unit, Dept Pharmacol Toxicol & Pharm, Merelbeke, Belgium

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 760卷 / 02期

关键词：

doping control; ephedrines;

D O I：

10.1016/S0378-4347(01)00275-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gas chromatographic method for the simultaneous quantitation of ephedrine, pseudoephedrine, norephedrine (phenyl-propanolamine), norpseudoephedrine (cathine) and methylephedrine in urine is described. The method consists of a liquid-liquid extraction with tert.-butyl methyl ether at pH 14. The extracts are analysed on a GC system equipped with an Rtx-5 Amine column and a nitro gen-phosphorus detector. Method validation shows excellent separation, linearity, specificity, accuracy, precision, intra-laboratory repeatability and reproducibility, making the method especially suitable for quantitation of ephedrines in urine samples for doping control purposes. A statistical analysis on the abuse of the different ephedrines in urine from athletes controlled in the Flemish doping control laboratory during the period 1993-2000 is included. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：255 / 261

页数：7

共 18 条

[1]

BARKAN S, 1988, J CHROMATOGR, V219, P81

[2] Validation of liquid chromatographic and gas chromatographic methods - Applications to pharmacokinetics [J].