Heterotypic gap junction channel formation between heteromeric and homomeric Cx40 and Cx43 connexons

被引:84
作者
Cottrell, GT [1 ]
Burt, JM [1 ]
机构
[1] Univ Arizona, Arizona Hlth Sci Ctr, Dept Physiol, Tucson, AZ 85724 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2001年 / 281卷 / 05期
关键词
connexins; intercellular communication; electrophysiology;
D O I
10.1152/ajpcell.2001.281.5.C1559
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Recent evidence indicating formation of functional homomeric/heterotypic gap junction channels by connexin40 (Cx40) and connexin43 (Cx43) raises the question of whether data previously interpreted as support for heteromeric channel formation by these connexins might not instead reflect the activity of homomeric/heterotypic channels. To address this question and to further characterize the behavior of these channels, we used dual whole cell voltage-clamp techniques to examine the junctions formed between cells that express only Cx40 (Rin40) or Cx43 (Rin43) and compared the results with those obtained when either of these cell types was paired with cells that naturally express both connexins (A7r5 cells). Rin40/Rin43 cell pairs formed functional gap junctions that displayed a strongly asymmetric voltage-dependent gating response. Single-channel event amplitudes ranged between 34 and 150 pS, with 90- to 130-pS events predominating. A7r5/Rin43 and A7r5/Rin40 cell pairs had voltage-dependent gating responses that varied greatly, with most pairs demonstrating strong asymmetry. These cell pairs exhibited a variety of single-channel events that were not consistent with homomeric/homotypic Cx40 or Cx43 channels or homomeric/heterotypic Cx40/Cx43 channels. These data indicate that Cx40 and Cx43 form homomeric/heterotypic as well as heteromeric/heterotypic channels that display unique gating and conductance properties.
引用
收藏
页码:C1559 / C1567
页数:9
相关论文
共 27 条
[1]   Connexin43 gap junctions exhibit asymmetrical gating properties [J].
Banach, K ;
Weingart, R .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1996, 431 (05) :775-785
[2]   GAP-JUNCTIONS FORMED BY CONNEXIN-26 AND CONNEXIN-32 ALONE AND IN COMBINATION ARE DIFFERENTLY AFFECTED BY APPLIED VOLTAGE [J].
BARRIO, LC ;
SUCHYNA, T ;
BARGIELLO, T ;
XU, LX ;
ROGINSKI, RS ;
BENNETT, MVL ;
NICHOLSON, BJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (19) :8410-8414
[3]   Connexin43 gap junction levels during development of the thoracic aorta are temporally correlated with elastic laminae deposition and increased blood pressure [J].
Blackburn, JP ;
Connat, JL ;
Severs, NJ ;
Green, CR .
CELL BIOLOGY INTERNATIONAL, 1997, 21 (02) :87-97
[4]   Evidence for heteromeric gap junction channels formed from rat connexin43 and human connexin37 [J].
Brink, PR ;
Cronin, K ;
Banach, K ;
Peterson, E ;
Westphale, EM ;
Seul, KH ;
Ramanan, SV ;
Beyer, EC .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 1997, 273 (04) :C1386-C1396
[5]   CONNEXIN40, A COMPONENT OF GAP-JUNCTIONS IN VASCULAR ENDOTHELIUM, IS RESTRICTED IN ITS ABILITY TO INTERACT WITH OTHER CONNEXINS [J].
BRUZZONE, R ;
HAEFLIGER, JA ;
GIMLICH, RL ;
PAUL, DL .
MOLECULAR BIOLOGY OF THE CELL, 1993, 4 (01) :7-20
[6]   BIOPHYSICAL PROPERTIES OF GAP JUNCTION CHANNELS FORMED BY MOUSE CONNEXIN40 IN INDUCED PAIRS OF TRANSFECTED HUMAN HELA-CELLS [J].
BUKAUSKAS, FF ;
ELFGANG, C ;
WILLECKE, K ;
WEINGART, R .
BIOPHYSICAL JOURNAL, 1995, 68 (06) :2289-2298
[7]   Alteration of Cx43:Cx40 expression ratio in A7r5 cells [J].
Burt, JM ;
Fletcher, AM ;
Steele, TD ;
Wu, Y ;
Cottrell, GT ;
Kurjiaka, DT .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2001, 280 (03) :C500-C508
[8]   SPECIFIC PERMEABILITY AND SELECTIVE FORMATION OF GAP JUNCTION CHANNELS IN CONNEXIN-TRANSFECTED HELA-CELLS [J].
ELFGANG, C ;
ECKERT, R ;
LICHTENBERGFRATE, H ;
BUTTERWECK, A ;
TRAUB, O ;
KLEIN, RA ;
HULSER, DF ;
WILLECKE, K .
JOURNAL OF CELL BIOLOGY, 1995, 129 (03) :805-817
[9]   Coexpression of connexins 40 and 43 enhances the pH sensitivity of gap junctions - A model for synergistic interactions among connexins [J].
Gu, H ;
Ek-Vitorin, JF ;
Taffet, SM ;
Delmar, M .
CIRCULATION RESEARCH, 2000, 86 (10) :E98-E103
[10]   Incompatibility of connexin 40 and 43 hemichannels in gap junctions between mammalian cells is determined by intracellular domains [J].
Haubrich, S ;
Schwarz, HJ ;
Bukauskas, F ;
LichtenbergFrate, H ;
Traub, O ;
Weingart, R ;
Willecke, K .
MOLECULAR BIOLOGY OF THE CELL, 1996, 7 (12) :1995-2006