In vivo and in absence of a thymus, the enforced expression of the notch ligands delta-1 or delta-4 promotes T cell development with specific unique effects

被引:32
作者
de La Coste, A
Six, E
Fazilleau, N
Mascarell, L
Legrand, N
Mailhé, MP
Cumano, A
Laâbi, Y
Freitas, AA
机构
[1] Inst Pasteur, Unite Biol Populat Lymphocytaires, F-75724 Paris 15, France
[2] Inst Pasteur, Unite Biol Mol Express Gen, CNRS, URA 2582, F-75724 Paris 15, France
[3] Inst Pasteur, Unite Rech & Expertise Immunite Anti Virale, F-75724 Paris 15, France
[4] Inst Pasteur, Unite Biol Regulat Immunitaires, F-75724 Paris 15, France
[5] Inst Pasteur, Unite Dev Lymphocytes, F-75724 Paris 15, France
关键词
D O I
10.4049/jimmunol.174.5.2730
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The role of Notch signaling in T cell commitment during lymphoid development is well established. However, the identity of the ligand that triggers this critical signal in vivo is still unclear. By overexpressing Delta-1 and Delta-4 ligands in the hemopoietic cells of athymic nu/nu host mice, we demonstrate that, in vivo and in the absence of a thymus, Delta-1 or Delta-4 expression is sufficient to promote T cell development from the most immature progenitor stages to complete maturation of both CD8(+) and CD4(+) alphabeta T cells. The mature T cells developing in a Delta-1- or Delta-4-enriched environment express a diverse TCR repertoire, are able to proliferate upon in vitro TCR stimulation, but show different profiles of cytokine production after in vitro anti-CD3 stimulation.
引用
收藏
页码:2730 / 2737
页数:8
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