Development and preliminary evaluation of a loop-mediated isothermal amplification procedure for sensitive detection of Cryptosporidium oocysts in fecal and water samples

被引：107

作者：

Karanis, Panagiotis

Thekisoe, Oriel

Kiouptsi, Klytaimnistra

Ongerth, Jerry

Igarashi, Ikuo

Inoue, Noborou

机构：

[1] Obihiro Univ Agr & Vet Med, Natl Res Ctr Protozoan Dis, Obihiro, Hokkaido 0808555, Japan

[2] Univ Cologne, Inst 2, Ctr Anat, Cologne, Germany

[3] Univ Wollongong, Wollongong, NSW 2522, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 17期

关键词：

D O I：

10.1128/AEM.01152-07

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

loop-mediated isothermal amplification (LAMP) procedure for the detection of Cryptosporidium in environmental and fecal samples was developed and evaluated. This is the first demonstration of LAMP applied to detection of Cryptosporidium. Due to its specificity and simplicity, the method could become a useful diagnostic tool for epidermiologic studies of Cryptosporidium presence.

引用

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页码：5660 / 5662

页数：3

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