Miranda mediates asymmetric protein and RNA localization in the developing nervous system

被引:203
作者
Schuldt, AJ
Adams, JHJ
Davidson, CM
Micklem, DR
Haseloff, J
St Johnston, D
Brand, AH [1 ]
机构
[1] Wellcome CRC Inst, Cambridge CB2 1QR, England
[2] Univ Cambridge, Dept Genet, Cambridge CB2 1QR, England
[3] MRC, Mol Biol Lab, Cambridge CB2 2QH, England
基金
英国惠康基金;
关键词
RNA localization; asymmetric segregation; Miranda; Staufen; Prospero; nervous system;
D O I
10.1101/gad.12.12.1847
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Neuroblasts undergo asymmetric stem cell divisions to generate a series of ganglion mother cells (GMCs). During these divisions, the cell fate determinant Prospero is asymmetrically partitioned to the GMC by Miranda protein, which tethers it to the basal cortex of the dividing neuroblast. Interestingly, prospero mRNA is similarly segregated by the dsRNA binding protein, Staufen. Here we show that Staufen interacts in vivo with a segment of the prospero 3' UTR. Staufen protein and prospero RNA colocalize to the apical side of the neuroblast at interphase, but move to the basal side during prophase. Both the apical and basal localization of Staufen are abolished by the removal of a conserved domain from the carboxyl terminus of the protein, which interacts in a yeast two-hybrid screen with Miranda protein. Furthermore, Miranda colocalizes with Staufen protein and prospero mRNA during neuroblast divisions, and neither Staufen nor prospero RNA are localized in miranda mutants. Thus Miranda, which localizes Prospero protein, also localizes prospero RNA through its interaction with Staufen protein.
引用
收藏
页码:1847 / 1857
页数:11
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