Platelet function measured by VerifyNow™ identifies generalized high platelet reactivity in aspirin treated patients

被引:45
作者
Dichiara, Joseph [1 ]
Bliden, Kevin P. [1 ]
Tantry, Udaya S. [1 ]
Chaganti, Srivasavi K. [1 ]
Kreutz, Rolf P. [1 ]
Gesheff, Tania B. [1 ]
Kreutz, Yvonne [1 ]
Gurbel, Paul A. [1 ]
机构
[1] Sinai Ctr Thrombosis Res, Baltimore, MD 21215 USA
关键词
VerifyNow; platelet reactivity; aspirin;
D O I
10.1080/09537100701206824
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Selected aspirin treated patients may exhibit high platelet reactivity to agonists other than arachidonic acid. This study aimed to determine whether the VerifyNow (TM) identifies generalized high platelet reactivity supported by correlations with other established methods that stimulate platelets with various agonists. Stable outpatients with coronary artery disease (n =110) were treated with aspirin in a two 3 x 3 Latin square design (81, 162 and 325 mg/day for 4 weeks each). VerifyNow (TM) (arachidonic acid (AA) cartridge); light transmittance aggregometry; thrombelastography; PFA-100((R)); flow cytometry; PlateletWorks((R)); and urinary 11-dehydro thromboxane levels were measured. Multianalyte profiling measured fibrinogen and von Willebrand factor (vWF). Patients with >= 550 ARU by VerifyNow (TM) had increased 5mM AA-, 5 mu M ADP-, and 2 mu g/mL collagen-induced platelet aggregation compared to patients with < 550 ARU (p <= 0.001). The highest ADP- and collagen-induced aggregation was present in the upper quartile by VerifyNow (TM) (p < 0.05). Fibrinogen or vWF levels did not differ between VerifyNow (TM) quartiles. Patients with high platelet reactivity to arachidonic acid identified by VerifyNow (TM) also exhibit increased reactivity to other important platelet agonists that is independent of fibrinogen and vWF levels. These data suggest that VerifyNow (TM) may identify a generalized high platelet reactivity phenotype.
引用
收藏
页码:414 / 423
页数:10
相关论文
共 35 条
[31]   Evaluation of the profile of thrombin generation during the process of whole blood clotting as assessed by thrombelastography [J].
Rivard, GE ;
Brummel-Ziedins, KE ;
Mann, KG ;
Fan, L ;
Hofer, A ;
Cohen, E .
JOURNAL OF THROMBOSIS AND HAEMOSTASIS, 2005, 3 (09) :2039-2043
[32]   ACETYLATION OF PROSTAGLANDIN SYNTHASE BY ASPIRIN [J].
ROTH, GJ ;
STANFORD, N ;
MAJERUS, PW .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (08) :3073-3076
[33]  
Tantry U, 2006, J AM COLL CARDIOL, V47, p290A
[34]   Overestimation of platelet aspirin resistance detection by thrombelastograph platelet mapping and validation by conventional aggregometry using arachidonic acid stimulation [J].
Tantry, US ;
Bliden, KP ;
Gurbel, PA .
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, 2005, 46 (09) :1705-1709
[35]   Incidence of aspirin nonresponsiveness using the Ultegra Rapid Platelet Function Assay-ASA [J].
Wang, JC ;
Aucoin-Barry, D ;
Manuelian, D ;
Monbouquette, R ;
Reisman, M ;
Gray, W ;
Block, PC ;
Block, EH ;
Ladenheim, M ;
Simon, DI .
AMERICAN JOURNAL OF CARDIOLOGY, 2003, 92 (12) :1492-1494