Novel factor XIII probes for blood coagulation imaging

被引:58
作者
Tung, CH [1 ]
Ho, NH
Tang, Q
Jaffer, FA
Reed, GL
Weissleder, R
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Ctr Mol Imaging Res, Charlestown, MA 02129 USA
[2] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Cardiol Div,Dept Med, Boston, MA 02115 USA
关键词
blood coagulation; factor; 13; fluorescent probes; imaging agents; transglutaminase;
D O I
10.1002/cbic.200300602
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The clot thickens: An α2-antiplasmin peptide fragment labeled with a near-infrared fluorochrome or a paramagnetic reporter (see figure) can be used for imaging blood clot formation. A thrombosis-specific probe was developed based on blood coagulation factor XIII transglutaminase but the approach could be extended to other transglutaminases involved in diseases such as cancer, neurodegenerative diseases and celiac disease. The use of near-infrared fluorescence avoids the difficulty of background absorbance encountered when light imaging is used for blood imaging.
引用
收藏
页码:897 / 899
页数:3
相关论文
共 20 条
[1]   Macrocyclic chelators with paramagnetic cations are internalized into mammalian cells via a HIV-tat derived membrane translocation peptide [J].
Bhorade, R ;
Weissleder, R ;
Nakakoshi, T ;
Moore, A ;
Tung, CH .
BIOCONJUGATE CHEMISTRY, 2000, 11 (03) :301-305
[2]   The role of transglutaminases in neurodegenerative diseases: overview [J].
Cooper, AJL ;
Jeitner, TM ;
Blass, JP .
NEUROCHEMISTRY INTERNATIONAL, 2002, 40 (01) :1-5
[3]   The vulnerable plaque and acute coronary syndromes [J].
Corti, R ;
Farkouh, ME ;
Badimon, JJ .
AMERICAN JOURNAL OF MEDICINE, 2002, 113 (08) :668-680
[4]   Real-time in vivo imaging of platelets, tissue factor and fibrin during arterial thrombus formation in the mouse [J].
Falati, S ;
Gross, P ;
Merrill-Skoloff, G ;
Furie, BC ;
Furie, B .
NATURE MEDICINE, 2002, 8 (10) :1175-1180
[5]   In vivo Imaging of thrombin activity in experimental thrombi with thrombin-sensitive near-infrared molecular probe [J].
Jaffer, FA ;
Tung, CH ;
Gerszten, RE ;
Weissleder, R .
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 2002, 22 (11) :1929-1935
[6]   Current strategies for the diagnosis of pulmonary embolus [J].
Johnson, MS .
JOURNAL OF VASCULAR AND INTERVENTIONAL RADIOLOGY, 2002, 13 (01) :13-23
[7]   Transglutaminase in disease [J].
Kim, SY ;
Jeitner, TM ;
Steinert, PM .
NEUROCHEMISTRY INTERNATIONAL, 2002, 40 (01) :85-103
[8]  
Lee KN, 2001, ANN NY ACAD SCI, V936, P335
[9]  
Lorand L, 2001, ANN NY ACAD SCI, V936, P291
[10]   Near-infrared optical imaging of protease activity for tumor detection [J].
Mahmood, U ;
Tung, CH ;
Bogdanov, A ;
Weissleder, R .
RADIOLOGY, 1999, 213 (03) :866-870