Substrate assisted catalysis application to G proteins

被引：36

作者：

Kosloff, M ^{[1
]}

Selinger, Z

机构：

[1] Hebrew Univ Jerusalem, Inst Life Sci, Dept Biol Chem, IL-91904 Jerusalem, Israel

[2] Hebrew Univ Jerusalem, Inst Life Sci, Kuhne Minerva Ctr, IL-91904 Jerusalem, Israel

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2001年 / 26卷 / 03期

关键词：

D O I：

10.1016/S0968-0004(00)01748-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The idea that both the substrate and the enzyme contribute to catalysis (substrate assisted catalysis; SAC) is applicable to guanine nucleotide-binding proteins (G proteins). Naturally occurring SAC uses GTP as a general base in the GTPase reaction catalyzed by G proteins. Engineered SAC has identified a putative rate-limiting step for the GTPase reaction a nd shown that GTPase-deficient oncogenic Ras mutants are not irreversibly impaired. Thus, anti-cancer drugs could potentially be designed to restore the blocked GTPase reaction.

引用

页码：161 / 166

页数：6

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