Chemistry of sulforhodamine-amine conjugates

被引：22

作者：

Corrie, JET ^{[1
]}

Davis, CT ^{[1
]}

Eccleston, JF ^{[1
]}

机构：

[1] Natl Inst Med Res, London NW7 1AA, England

来源：

BIOCONJUGATE CHEMISTRY | 2001年 / 12卷 / 02期

关键词：

D O I：

10.1021/bc0000785

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Commercially-available sulforhodamine sulfonyl chlorides contain two isomeric monosulfonyl chlorides. Conjugates of these isomers with amines have different properties because the sulfonamide formed from one isomer can undergo ring-closure to a colorless sultam. This chemistry has been examined for a model conjugate with methylamine and for a bioconjugate with 2'(3')-O-[N-(2-aminoethyl)carbamoyl]ATP. The interaction of each isomer of the latter conjugates with myosin subfragment 1 has been characterized. Significant differences between the two isomers are observed in these interactions.

引用

页码：186 / 194

页数：9

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