Enhancement of monocyte procoagulant activity by adhesion on vascular smooth muscle cells and intercellular adhesion molecule-1-transfected Chinese hamster ovary cells

被引:23
作者
Marx, N
Neumann, FJ
Zohlnhöfer, D
Dickfeld, T
Fischer, A
Heimerl, S
Schömig, A
机构
[1] Tech Univ Munchen, Med Klin 1, D-80636 Munchen, Germany
[2] Tech Univ Munchen, Deutsch Herzzentrum, D-80636 Munchen, Germany
关键词
muscle; smooth; cells; cell adhesion molecules; coagulation; tissue;
D O I
10.1161/01.CIR.98.9.906
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background-Plaque erosion is a frequent finding in sudden death due to coronary thrombosis. The present study sought to investigate whether monocyte adhesion to human aortic vascular smooth muscle cells (VSMCs) induces procoagulant activity (PCA) and whether this could be mediated by intercellular adhesion molecule-1 (ICAM-1). Methods and Results-We incubated mononuclear cells (MNCs) with VSMCs and ICAM-1-transfected Chinese hamster ovary (CHO) cells, investigated monocyte tissue factor (TF) mRNA expression by Northern blot analysis and TF protein expression by ELISA, and measured PCA. Incubation of MNCs with VSMCs for 6 hours increased PCA from 0.7+/-0.1 to 166.0+/-37.9 mU/10(5) cells (P=0.007), which could be inhibited in a dose-dependent manner by the addition of blocking anti-ICAM-1 monoclonal antibodies. Prestimulation of VSMCs with interleukin-1 beta enhanced surface ICAM-1 expression significantly but did not induce PCA in VSMCs. Incubation of MNCs with prestimulated VSMCs led to a further increase in PCA to 239.9+/-27.9 mU/10(5) cells (P=0.02 compared with incubation with unstimulated VSMCs). Incubation of MNCs with VSMCs enhanced TF mRNA after 2 hours and significantly increased TF protein content after 6 hours. Incubation of purified monocytes with ICAM-1-transfected CHO cells increased PCA from 1.2+/-0.2 to 81.9+/-3.3 mU/10(5) cells (P<0.001 compared with incubation with untransfected CHO cells) after 6 hours. This effect could be inhibited significantly by the addition of blocking anti-CD18, anti-CD11b, or anti-CD11c monoclonal antibodies. Similar results were obtained for MNCs. Conclusions-Monocyte adhesion to VSMCs induces TF nRNA and protein expression and monocyte PCA, which is regulated by beta(2)-integrin-mediated monocyte adhesion to ICAM-1 on VSMCs.
引用
收藏
页码:906 / 911
页数:6
相关论文
共 30 条
[1]   RECOMBINANT TUMOR-NECROSIS-FACTOR INDUCES PROCOAGULANT ACTIVITY IN CULTURED HUMAN VASCULAR ENDOTHELIUM - CHARACTERIZATION AND COMPARISON WITH THE ACTIONS OF INTERLEUKIN-1 [J].
BEVILACQUA, MP ;
POBER, JS ;
MAJEAU, GR ;
FIERS, W ;
COTRAN, RS ;
GIMBRONE, MA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (12) :4533-4537
[2]   A monoclonal antibody, 3/22, to rabbit CD11c which induces homotypic T cell aggregation: Evidence that ICAM-1 is a ligand for CD11c/CD18 [J].
Blackford, J ;
Reid, HW ;
Pappin, DJC ;
Bowers, FS ;
Wilkinson, JM .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (03) :525-531
[3]   Coronary risk factors and plaque morphology in men with coronary disease who died suddenly [J].
Burke, AP ;
Farb, A ;
Malcom, GT ;
Liang, YH ;
Smialek, J ;
Virmani, R .
NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (18) :1276-1282
[4]   SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].
CHOMCZYNSKI, P ;
SACCHI, N .
ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159
[5]   INDUCTION OF TISSUE FACTOR EXPRESSION IN HUMAN MONOCYTE/ENDOTHELIUM COCULTURES [J].
COLLINS, PW ;
NOBLE, KE ;
REITTIE, JR ;
HOFFBRAND, AV ;
PASI, KJ ;
YONG, KL .
BRITISH JOURNAL OF HAEMATOLOGY, 1995, 91 (04) :963-970
[6]   REGULATION OF VASCULAR CELL-ADHESION MOLECULE-1 AND INTERCELLULAR-ADHESION MOLECULE-1 IN HUMAN VASCULAR SMOOTH-MUSCLE CELLS [J].
COUFFINHAL, T ;
DUPLAA, C ;
MOREAU, C ;
LAMAZIERE, JMD ;
BONNET, J .
CIRCULATION RESEARCH, 1994, 74 (02) :225-234
[7]   The composition of coronary-artery plaques [J].
Davies, MJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1997, 336 (18) :1312-1314
[8]   Stability and instability: Two faces of coronary atherosclerosis - The Paul Dudley White Lecture 1995 [J].
Davies, MJ .
CIRCULATION, 1996, 94 (08) :2013-2020
[9]   BINDING OF THE INTEGRIN MAC-1 (CD11B/CD18) TO THE 3RD IMMUNOGLOBULIN-LIKE DOMAIN OF ICAM-1 (CD54) AND ITS REGULATION BY GLYCOSYLATION [J].
DIAMOND, MS ;
STAUNTON, DE ;
MARLIN, SD ;
SPRINGER, TA .
CELL, 1991, 65 (06) :961-971
[10]   COUPLING OF THE ADHESIVE RECEPTOR CD11B/CD18 TO FUNCTIONAL ENHANCEMENT OF EFFECTOR MACROPHAGE TISSUE FACTOR RESPONSE [J].
FAN, ST ;
EDGINGTON, TS .
JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (01) :50-57