Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets

被引:61
作者
Mirandola, Prisco
Gobbi, Giuliana
Sponzilli, Ivonne
Pambianco, Maurizia
Malinverno, Chiara
Cacchioli, Antonio
De Panfilis, Giuseppe
Vitale, Marco
机构
[1] Univ Parma, Human Anat Sect, Dept Anat Pharmacol & Forens Med, Osped Maggiore, I-43100 Parma, Italy
[2] Cell Biol Lab, Brescia, Italy
[3] Univ Parma, Dept Anim Hlth, I-43100 Parma, Italy
[4] Univ Parma, Dermatol Sect, Dept Surg, I-43100 Parma, Italy
关键词
D O I
10.1002/jcp.21151
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The toxic effects of exogenous hydrogen sulfide on peripheral blood lymphocytes have been investigated in detail. Hydrogen sulfide is now considered as a gasotransmitter with specific functional roles in different cell types, like neurons and vascular smooth muscle. Here we show that exogenous hydrogen sulfide induces a caspase-independent cell death of peripheral blood lymphocytes that depends on their intracellular glutathion levels, with a physiologically relevant subset specificity for CD8(+) T cells and NK cells. Although lymphocyte activation does not modify their sensitivity to HS-, after 24 h exposure to hydrogen sulfide surviving lymphocyte subsets show a dramatically decreased proliferation in response to mitogens and a reduced IL-2 production. Overall, our data demonstrate that HS(-)reduces the cellular cytotoxic response of peripheral blood lymphocytes as well as their production of IL-2, therefore de-activating the major players of local inflammatory responses, adding new basic knowledge to the clinically well known anti-inflammatory effects of sulfur compounds.
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页码:826 / 833
页数:8
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