The cellular antioxidant activity in red blood cells (CAA-RBC): A new approach to bioavailability and synergy of phytochemicals and botanical extracts

被引:68
作者
Blasa, Manuela [1 ]
Angelino, Donato [1 ]
Gennari, Lorenzo [1 ]
Ninfali, Paolino [1 ]
机构
[1] Univ Urbino Carlo Bo, Dept Biomol Sci, Urbino, Italy
关键词
Flavonoids; Botanical extracts; Cellular antioxidant activity; ORAC; Erythrocytes; Phytochemicals synergy; Bioavailability; AAPH-INDUCED HEMOLYSIS; IN-VIVO ANTIOXIDANT; DIETARY-SUPPLEMENTS; ALPHA-TOCOPHEROL; CAPACITY; ERYTHROCYTES; FOODS; ASSAY; PEROXIDATION; NUTRITION;
D O I
10.1016/j.foodchem.2010.09.065
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In the present work, human red blood cells (RBC) were used to determine cellular antioxidant activity (CAA-RBC) of pure phytochemicals and botanical extracts, with the aim to predict their bioavailability. Amongst the pure flavonoids, isorhamnetin, tamarixetin, myricetin, and kaempferol showed the highest activity in the CAA-RBC assay; whereas, with the "chemical" oxygen radical absorbance capacity (ORAC) assay, the compounds that showed the highest activity were isorhamnetin, resveratrol, apigenin and catechin. When the CAA-RBC assay was applied to herbal extracts, the Vitis vinifera showed the highest value, a position that this extract maintained also when the ORAC assay was used. Other extracts showed a different order of effectiveness with the two methods. We also employed the CAA-RBC to assess synergistic or antagonistic effects of combinations of herbal extracts and we again compared the results with the ORAC assay. Punica granatum + Malus domestica synergized in the CAA-RBC assay, but not in the ORAC assay; Aspalathus linearis extract interacted positively with Vaccinium myrtillus, both in the ORAC assay and in the CAA-RBC assay. We concluded that the CAA-RBC assay, coupled with the ORAC assay, was useful for evaluating intracellular bioactivity and synergy amongst phytochemicals or extracts. (C) 2010 Elsevier Ltd All rights reserved.
引用
收藏
页码:685 / 691
页数:7
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