RETRACTED: Virus-induced gene silencing for functional analysis of selected genes (Retracted Article. See vol 28, pg 335, 2009)

被引：24

作者：

Godge, Mandar R. ^{[1
]}

Purkayastha, Arunima ^{[2
]}

Dasgupta, Indranil ^{[2
]}

Kumar, Prakash P. ^{[1
]}

机构：

[1] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 117543, Singapore

[2] Univ Delhi, Dept Plant Mol Biol, New Delhi 110021, India

来源：

PLANT CELL REPORTS | 2008年 / 27卷 / 02期

关键词：

VIGS; viral vectors; gene silencing; functional genomics;

D O I：

10.1007/s00299-007-0460-2

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity, robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.

引用

页码：209 / 219

页数：11

共 77 条

[1] Simultaneous suppression of multiple genes by single transgenes.: Down-regulation of three unrelated lignin biosynthetic genes in tobacco [J].

Abbott, JC ;

Barakate, A ;

Pinçon, G ;

Legrand, M ;

Lapierre, C ;

Mila, I ;

Schuch, W ;

Halpin, C .

PLANT PHYSIOLOGY, 2002, 128 (03) :844-853

[2]

Ahn CS, 2005, MOL CELLS, V20, P112

[3] Transcriptional and posttranscriptional plant gene silencing in response to a pathogen [J].

Al-Kaff, NS ;

Covey, SN ;

Kreike, MM ;

Page, AM ;

Pinder, R ;

Dale, PJ .

SCIENCE, 1998, 279 (5359) :2113-2115

[4] Fast forward genetics based on virus-induced gene silencing [J].

Baulcombe, DC .

CURRENT OPINION IN PLANT BIOLOGY, 1999, 2 (02) :109-113

[5] Virus-induced gene silencing in Solanum species [J].

Brigneti, G ;

Martín-Hernández, AM ;

Jin, HL ;

Chen, J ;

Baulcombe, DC ;

Baker, B ;

Jones, JDG .

PLANT JOURNAL, 2004, 39 (02) :264-272

[6]

Brunt A.A., 1996, Plant viruses online

[7] Efficient virus-induced gene silencing in Arabidopsis [J].

Burch-Smith, Tessa M. ;

Schiff, Michael ;

Liu, Yule ;

Dinesh-Kumar, S. P. .

PLANT PHYSIOLOGY, 2006, 142 (01) :21-27

[8] Applications and advantages of virus-induced gene silencing for gene function studies in plants [J].

Burch-Smith, TM ;

Anderson, JC ;

Martin, GB ;

Dinesh-Kumar, SP .

PLANT JOURNAL, 2004, 39 (05) :734-746

[9] Development of a virus-induced gene-silencing system for functional analysis of the RPS2-dependent resistance signalling pathways in Arabidopsis [J].

Cai, Xin-Zhong ;

Xu, Qiu-Fang ;

Wang, Chang-Chun ;

Zheng, Zhong .

PLANT MOLECULAR BIOLOGY, 2006, 62 (1-2) :223-232

[10] Silencing a prohibitin alters plant development and senescence [J].

Chen, JC ;

Jiang, CZ ;

Reid, MS .

PLANT JOURNAL, 2005, 44 (01) :16-24

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