Detection and determination of anabolic steroids in nutritional supplements

被引：81

作者：

De Cock, KJS ^{[1
]}

Delbeke, FT ^{[1
]}

Van Eenoo, P ^{[1
]}

Desmet, N ^{[1
]}

Roels, K ^{[1
]}

De Backer, P ^{[1
]}

机构：

[1] Ghent Univ, Fac Vet Sci, Dept Pharmacol Toxicol & Pharm, Doping Control Unit, B-9820 Merelbeke, Belgium

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2001年 / 25卷 / 5-6期

关键词：

nutritional supplement; anabolic steroids; norandrostenedione; detection; excretion;

D O I：

10.1016/S0731-7085(01)00396-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method is described fur the determination of anabolic steroids including testosterone, 19-nor-4-androstene-3,17-dione. 4-androstene-3,17-dione and nandrolone in food supplements. Initial clean-up is done by HPLC followed by determination with GC/MS. A 'contaminated' food supplement was analysed and appeared to contain 19-nor-4-androstene-3,17-dione and 4-androstene-3,17-dione. One capsule of this nutritional supplement was ingested by five: male volunteers. Urine samples were collected and analysed by GC,:MS and GC,MS-MS. Neither the ratio testosterone/epitestosterone, nor the ratio androstenedione/epitestosterone increased significantly:. Concentrations above 2 ng/ml for norandrosterone. the major metabolite of nandrolone. were detected until 48-144 h after ingestion of the food supplement. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：843 / 852

页数：10

共 7 条

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