The Dose-Effect of Icariin on the Proliferation and Osteogenic Differentiation of Human Bone Mesenchymal Stem Cells

被引：119

作者：

Fan, Jun-Jun ^{[1
]}

Cao, Liang-Guo ^{[2
]}

Wu, Tao ^{[2
]}

Wang, De-Xin ^{[1
]}

Jin, Dan ^{[2
]}

Jiang, Shan ^{[2
]}

Zhang, Zhi-Yong ^{[1
]}

Bi, Long ^{[1
]}

Pei, Guo-Xian ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xi Jing Hosp, Dept Orthopaed Surg, Xian 710032, Peoples R China

[2] So Med Univ, Southern Hosp, Dept Orthopaed Surg, Guangzhou 510515, Guangdong, Peoples R China

来源：

MOLECULES | 2011年 / 16卷 / 12期

关键词：

icariin; dose-effect; human bone mesenchymal stem cells; osteogenic differentiation; proliferation; MARROW STROMAL CELLS; TRICALCIUM PHOSPHATE/CHITOSAN; OSTEOBLASTS; SCAFFOLDS;

D O I：

10.3390/molecules161210123

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Icariin had been reported as a potential agent for osteogenesis, but the dose-effect relationship needed further research to realize the clinical application of icariin. We isolated and purified human bone mesenchymal stem cells (hBMSCs) and stimulated them with different concentrations of icariin. The cytotoxicity of icariin was evaluated by the methylthiazolytetrazolium (MTT) assay method. The proliferation and osteogenic differentiation of such hBMSCs were investigated for different concentrations of icariin. We found that icariin had a dose-dependent effect on the proliferation and osteogenic differentiation of hBMSCs in a suitable concentration range from 10(-9) M to 10(-6) M, but at concentrations above 10(-5) M, the cytotoxicity limited its use. The extremely low cost of icariin and its high abundance make it appealing for bone regeneration.

引用

页码：10123 / 10133

页数：11

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