Effects of perorally applied endotoxin on colonic mucins of germfree rats

被引:28
作者
Enss, ML
Muller, H
SchmidtWittig, U
Kownatzki, R
Coenen, M
Hedrich, HJ
机构
[1] HANNOVER MED SCH, ANIM FACIL, D-30623 HANNOVER, GERMANY
[2] VET SCH HANOVER, INST ANIM NUTR, HANNOVER, GERMANY
[3] HANNOVER MED SCH, DEPT BIOPHYS CHEM, D-3000 HANNOVER, GERMANY
关键词
endotoxin; lipopolysaccharide; mucin; mucus;
D O I
10.3109/00365529609051994
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: The intestinal epithelium, with the potential to restrict luminal noxae from the host, secretes a mucous layer with various protective functions. Microbial colonization of germfree (GF) rats stimulates this mucin-secreting tissue. The present study determined the effect of bacterial lipopolysaccharides (LPS) on this process. Methods: One, 3, and 5 days after peroral application of 35 mu g LPS/100g body weight (from Escherichia coli 055:B5), LPS concentrations were monitored in ingesta, intestinal tissue, and liver. Mucin high molecular weight glycoproteins (HMG), released in response to LPS, were isolated and separated into mucins, i) attached to the colonic epithelium (EM) and ii mixed to the luminal content (LM), respectively. Subsequently, the binding capacity of both mucin fractions for various lectins and for type-1 pill expressing E. coli was determined. Results: Ingesta and tissue had maximal LPS concentrations on days 3 (jejunum) and 5 (colon). Maximal EM secretion was found on day 3, release of LM further increased to day 5. Both mucin fractions had altered glycosylation patterns: augmentation of beta-galactose, alpha-N-acetyl galactosamine, and mannose coincided with a decrease in alpha-fucose. Compared with the controls, attachment of E. coli to EM increased slightly on day 1 only; the binding capacity of LM increased continuously up to day 5. Conclusion: Results suggest that mucins, released in response to LPS, in addition to the epithelial protection, support the gut microbial clearance system.
引用
收藏
页码:868 / 874
页数:7
相关论文
共 46 条
[1]  
ABRAMS GD, 1963, LAB INVEST, V12, P356
[2]   ADHERENT AND SOLUBLE MUCUS IN THE STOMACH AND DUODENUM [J].
ALLEN, A ;
CARROLL, NJH .
DIGESTIVE DISEASES AND SCIENCES, 1985, 30 (11) :S55-S62
[3]   THE EFFECT OF DEXAMETHASONE AND ENDOTOXIN ADMINISTRATION ON BILIARY IGA AND BACTERIAL ADHERENCE [J].
ALVERDY, JC ;
AOYS, E .
JOURNAL OF SURGICAL RESEARCH, 1992, 53 (05) :450-454
[4]  
CARLSTEDT I, 1985, ESSAYS BIOCHEM, V20, P40
[5]   ENDOTOXIN-INDUCED ALTERATIONS IN RAT COLONIC WATER AND ELECTROLYTE TRANSPORT [J].
CIANCIO, MJ ;
VITIRITTI, L ;
DHAR, A ;
CHANG, EB .
GASTROENTEROLOGY, 1992, 103 (05) :1437-1443
[6]   INTERLEUKIN-1 IS A MUCUS SECRETAGOGUE [J].
COHAN, VL ;
SCOTT, AL ;
DINARELLO, CA ;
PRENDERGAST, RA .
CELLULAR IMMUNOLOGY, 1991, 136 (02) :425-434
[7]  
DEFAUX JP, 1993, J LIPID MEDIATOR, V7, P11
[8]  
DEITCH EA, 1989, SURGERY, V106, P292
[9]  
DINARELLO CA, 1994, EUR CYTOKINE NETW, V5, P517
[10]   PROSTAGLANDIN E(2) ALTERS TERMINAL GLYCOSYLATION OF HIGH-MOLECULAR-WEIGHT GLYCOPROTEINS, RELEASED BY PIG GASTRIC MUCOUS CELLS IN-VITRO [J].
ENSS, ML ;
SCHMIDTWITTIG, U ;
HEIM, HK ;
SEWING, KF .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1995, 52 (05) :333-340