Characterization of collagen matrices crosslinked using microbial transglutaminase

被引：108

作者：

Chen, RN ^{[1
]}

Ho, HO ^{[1
]}

Sheu, MT ^{[1
]}

机构：

[1] Taipei Med Univ, Coll Pharm, Grad Inst Pharmaceut Sci, Taipei 110, Taiwan

来源：

BIOMATERIALS | 2005年 / 26卷 / 20期

关键词：

transglutaminase; collagen; matrix; enzyme; crosslinking; biomaterials;

D O I：

10.1016/j.biomaterials.2004.11.012

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

In search of a new approach for crosslinking collagen-based biomaterials, we examined the effect of microbial transglutaminase (MTGases) as a crosslinking reagent on collagenous matrices made from porcine type I collagen. As the results revealed, MTGase exhibited a crosslinking action that raised the viscosity of the collagen solution. Matrices crosslinked with MTGase at the low pH values of pH 3 and 4 exhibited higher tensile strengths than those at high pH values. In comparison with untreated matrices, the denaturation temperatures of the corresponding matrices shifted toward higher temperatures. These enzyme-catalyzed crosslinked matrices were proven by MTT assay to be non-cytotoxic. In conclusion, this enzymatic method of using MTGase provides an alternative potential way for crosslinking collagen-based matrices.(C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：4229 / 4235

页数：7

共 34 条

[1]

Billiar K, 2001, J BIOMED MATER RES, V56, P101, DOI 10.1002/1097-4636(200107)56:1<101::AID-JBM1074>3.0.CO

[2]

2-6

[3]

THE DETERMINATION OF EPSILON-AMINO GROUPS IN SOLUBLE AND POORLY SOLUBLE PROTEINACEOUS MATERIALS BY A SPECTROPHOTOMETRIC METHOD USING TRINITROBENZENESULFONIC ACID [J].

BUBNIS, WA ;

OFNER, CM .

ANALYTICAL BIOCHEMISTRY, 1992, 207 (01) :129-133

[4]

Enzyme-catalyzed gel formation of gelatin and chitosan: potential for in situ applications [J].

Chen, TH ;

Embree, HD ;

Brown, EM ;

Taylor, MM ;

Payne, GF .

BIOMATERIALS, 2003, 24 (17) :2831-2841

[5]

New gelatin-based hydrogels via enzymatic networking [J].

Crescenzi, V ;

Francescangeli, A ;

Taglienti, A .

BIOMACROMOLECULES, 2002, 3 (06) :1384-1391

[6]

TRANSGLUTAMINASES [J].

FOLK, JE .

ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 :517-531

[7]

Effects of processing conditions on the theological behavior of collagen dispersions [J].

Friess, W ;

Schlapp, M .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2001, 51 (03) :259-265

[8]

Influence of gelatin matrices cross-linked with transglutaminase on the properties of an enclosed bioactive material using beta-galactosidase as model system [J].

Fuchsbauer, HL ;

Gerber, U ;

Engelmann, J ;

Seeger, T ;

Sinks, C ;

Hecht, T .

BIOMATERIALS, 1996, 17 (15) :1481-1488

[9]

Biocompatibility studies of anionic collagen membranes with different degree of glutaraldehyde cross-linking [J].

Goissis, G ;

Junior, EM ;

Marcantônio, JAC ;

Lia, RCC ;

Cancian, DCJ ;

de Carvalho, M .

BIOMATERIALS, 1999, 20 (01) :27-34

[10]

Glutaraldehyde as a fixative in bioprostheses and drug delivery matrices [J].

Jayakrishnan, A ;

Jameela, SR .

BIOMATERIALS, 1996, 17 (05) :471-484

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