Tepidimonas aquatica sp nov., a new slightly thermophilic β-proteobacterium isolated from a hot water tank

被引:22
作者
Freitas, M
Rainey, FA
Nobre, MF
Silvestre, AJD
da Costa, MS [1 ]
机构
[1] Univ Coimbra, Dept Zool, Dept Bioquim, P-3001401 Coimbra, Portugal
[2] Univ Coimbra, Dept Zool, Ctr Neurociencias, P-3001401 Coimbra, Portugal
[3] Louisiana State Univ, Dept Sci Biol, Baton Rouge, LA USA
[4] Univ Coimbra, Dept Zool, Coimbra, Portugal
[5] Univ Aveiro, Dept Quim, Aveiro, Portugal
基金
美国国家科学基金会;
关键词
Tepidimonas aquatica; beta-subclass; Proteobacteria; slightly thermophilic;
D O I
10.1078/072320203322497400
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
A bacterial isolate, with an optimum growth temperature of about 50 degreesC, was recovered from a domestic hot water tank in Coimbra. Phylogenetic analysis using 16S rRNA gene sequence indicated that strain CLN-1(T) is a member of the P-Proteo bacteria and represents a new species of the genus Tepidimonas. The major fatty acids of strain CLN-1(T) are 16:0, 17:0 cyclo and 16:1 omega7c. Ubiquinone 8 is the major respiratory quinone, the major polar lipids are phosphatidylethanolamine, and phosphatidylglycerol. The new isolate is aerobic and facultatively chemolithoheterotrophic. Thiosulfate and tetrathionate are oxidized to sulfate in the presence of a metabolizable carbon source. Strain CLN-1(T) grows on amino acids and organic acids, but this organism does not assimilate carbohydrates. Glycerol is the only polyol assimilated. Resinic acids, namely abietic acid, dehydroabietic acid and isopimaric acid are not degraded. On the basis of the phylogenetic analyses, physiological and biochemical characteristics, we propose that strain CLN-1(T) represents a new species for which we offer the name Tepidimonas aquatica.
引用
收藏
页码:376 / 381
页数:6
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