Fabrication of calcium fluoride capillary electrophoresis microdevices for on-chip infrared detection

被引：39

作者：

Pan, T ^{[1
]}

Kelly, RT ^{[1
]}

Asplund, MC ^{[1
]}

Woolley, AT ^{[1
]}

机构：

[1] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2004年 / 1027卷 / 1-2期

关键词：

detection; electrophoresis; chip technology; instrumentation; calcium fluoride; amino acids;

D O I：

10.1016/S0021-9673(03)01300-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In this paper, we demonstrate microfluidic capillary electrophoresis (CE) devices made in CaF2, for optical detection in a broad spectral range. We have desiged methods for micromachining and enclosing capillaries in CaF2. The utility of these microdevices has been shown through CE analysis of fluorescently labeled amino acids. We have also performed infrared spectroscopy for analyte identification in microfluidic CaF2 channels. These CaF2 microdevices open the door to microchip separations with optical detection in the ultraviolet, visible, and infrared spectral regions. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：231 / 235

页数：5

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