3-nitroadipate, a metabolic intermediate for mineralization of 2,4-dinitrophenol by a new strain of a Rhodococcus species

The bacterial strain RB1 has been isolated by enrichment cultivation with 2,3-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16S rRNA gene sequence comparison, as a Rhodococcus species closely related to Rhodococcus opacus, Rhodococcus sp, strain RB1 degrades 2,4dinitrophenol, releasing the two nitro groups from the compound as nitrite, The release of nitro groups from 2,4-dinitrophenol occurs in two steps. First, the 2-nitro group is removed as nitrite, with the production of an aliphatic nitro compound identified by H-1 nuclear magnetic resonance and mass spectrometry as 3-nitroadipate. Then, this metabolic derivative is further metabolized, releasing its nitro group as nitrite, Full nitrite assimilation upon reduction to ammonia requires that an additional carbon source be supplied to the medium.