Detection of Helicobacter pylori associated with zooplankton

被引：21

作者：

Cellini, L

Di Campli, E

Grande, R

Di Bartolomeo, S

Prenna, M

Pasquantonio, MS

Pane, L

机构：

[1] Univ G DAnnunzio, Dept Biomed Sci, I-66100 Chieti, Italy

[2] Univ Camerino, Dept Biol MCA, I-62032 Camerino, Italy

[3] Univ Genoa, Dept Expt Environm & Appl Biol, I-16100 Genoa, Italy

来源：

AQUATIC MICROBIAL ECOLOGY | 2005年 / 40卷 / 02期

关键词：

Helicobacter pylori; zooplanktonic organisms; transmission; seawater; viable-butnon-culturable (VBNC) state; Tigriopus fulvus;

D O I：

10.3354/ame040115

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Helicobacter pylori was isolated from marine zooplankton, and characterized by standard microbiological tests, by PCR amplification of vacA and cagA gene fragments, and by comparative sequence analysis of the vacA PCR product. In a viable-but-non-culturable (VBNC) state, this isolate, as well as the reference strain H. pylori ATCC 43629, could be re-activated only when incubated in the presence of the marine copepod Tigriopus fulvus, and not in its absence. Isolate and type strain of H. pylori were found to be associated to the surface of T fulvus, which supports speculations about a potential role of copepods in H. pylori survival and transmission.

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收藏

页码：115 / 120

页数：6

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