Cytotoxic activity of G3 PAMAM-NH2 dendrimer-chlorambucil conjugate in human breast cancer cells

被引：43

作者：

Bielawski, Krzysztof ^{[1
]}

Bielawska, Anna ^{[1
]}

Muszynska, Anna ^{[1
]}

Poplawska, Bozena ^{[1
]}

Czarnomysy, Robert ^{[1
]}

机构：

[1] Med Univ Bialystok, Dept Med Chem, PL-15089 Bialystok, Poland

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2011年 / 32卷 / 03期

关键词：

Breast cancer; Chlorambucil; PAMAM dendrimer; Collagen biosynthesis; DRUG-DELIVERY; APOPTOSIS; ASSAY; SERA;

D O I：

10.1016/j.etap.2011.08.002

中图分类号：

X [环境科学、安全科学];

学科分类号：

083001 [环境科学];

摘要：

Evaluation of the cytotoxicity of a novel G3 PAMAM-NH2 dendrimer-chlorambucil conjugate employing a MTT assay and inhibition of [H-3]thymidine incorporation into DNA in both MDA-MB-231 and MCF-7 breast cancer cells demonstrated that the conjugate was more potent antiproliferative agent than chlorambucil. It was found that dendrimer-chlorambucil conjugate was more active inhibitor of collagen biosynthesis than chlorambucil. Our experiments carried out with flow cytometry assessment of annexin V binding and fluorescent microscopy assay revealed that PAMAM-CH conjugate inhibited the proliferation of MCF-7 and MDA-231 malignant cells by increasing the number of apoptotic and necrotic cells. The apoptotic effect of PAMAM-CH conjugate was found to be stronger than that caused by chlorambucil. (C) 2011 Elsevier B.V. All rights reserved.

引用

页码：364 / 372

页数：9

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