High-throughput quantification of hydroxyproline for determination of collagen

被引：103

作者：

Hofman, Kathleen ^{[1
]}

Hall, Bronwyn ^{[1
]}

Cleaver, Helen ^{[1
]}

Marshall, Susan ^{[1
]}

机构：

[1] New Zealand Inst Plant & Food Res Ltd, Nat Extracts Unit, Port Nelson 7043, Nelson, New Zealand

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 417卷 / 02期

关键词：

Collagen; Hydroxyproline; Colorimetric assay; TISSUES; ACID;

D O I：

10.1016/j.ab.2011.06.019

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

An accurate and high-throughput assay for collagen is essential for collagen research and development of collagen products. Hydroxyproline is routinely assayed to provide a measurement for collagen quantification. The time required for sample preparation using acid hydrolysis and neutralization prior to assay is what limits the current method for determining hydroxyproline. This work describes the conditions of alkali hydrolysis that, when combined with the colorimetric assay defined by Woessner, provide a high-throughput, accurate method for the measurement of hydroxyproline. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：289 / 291

页数：3

共 8 条

[1]

FUNCTION OF HYDROXYPROLINE IN COLLAGENS [J].