Modification of hydrophilicity/hydrophobicity of the microenvironment of lipase of Candida rugosa by dextrans

被引：18

作者：

de la Casa, RM ^{[1
]}

Sánchez-Montero, JM ^{[1
]}

Sinisterra, JV ^{[1
]}

机构：

[1] Univ Complutense, Dept Organ & Pharmaceut Chem, E-28040 Madrid, Spain

来源：

BIOTECHNOLOGY LETTERS | 1999年 / 21卷 / 02期

关键词：

Candida rugosa; lipases; dextrans; water activity;

D O I：

10.1023/A:1005426101080

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Replacing the lactose used in the commercial preparation of lipase from Candida rugosa by dextrans with different molecular weight, several preparations with enhanced activities in esterification of (R,S)-ibuprofen in organic medium were obtained. The presence of carbohydrates modifies the microenvironment of the enzyme and maintains the hydration of the biocatalyst. We can modulate the hydrophilic/hydrophobic balance on the surface of the biocatalyst creating non covalent enzyme-dextran complexes.

引用

页码：123 / 128

页数：6

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