A novel function of transcription factor α-Pal/NRF-1:: Increasing neurite outgrowth

被引：28

作者：

Chang, WT

Chen, H

Chiou, RJ

Chen, CY

Huang, AM ^{[1
]}

机构：

[1] Natl Cheng Kung Univ, Coll Med, Inst Basic Med Sci, Tainan 701, Taiwan

[2] Natl Cheng Kung Univ, Coll Med, Dept Physiol, Tainan 701, Taiwan

[3] Natl Cheng Kung Univ, Coll Med, Dept Life Sci, Tainan 701, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 334卷 / 01期

关键词：

alpha-Pal/NRF-1; IAP/CD47; neurite outgrowth; dominant-negative mutant; serum deprivation; antisense cDNA; neuroblastoma; primary cortical neurons;

D O I：

10.1016/j.bbrc.2005.06.079

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

alpha-Pal/NRF-1 is a critical regulator of the promoter of human IAP/CD47 gene, a gene related to memory formation in rodents. However, its function in neurons was unknown. We found that stable or transient expression of full-length alpha-Pal/NRF-1 in human neuroblastoma IMR-32 cells significantly induced neurite outgrowth and increased the length of neurites both in medium containing 10% fetal bovine serum and in serum-free medium. In contrast, the dominant-negative mutant of alpha-Pal/NRF-1 inhibited the induction and extension of neurites. Ectopic expression of full-length alpha-Pal/NRF-1 also increased the induction of neurite outgrowth in primary mouse cortical neurons. The IAP antisense cDNA significantly inhibited the increase of neurite outgrowth by alpha-Pal/NRF-1. These findings indicate that a novel function of alpha-Pal/NRF-1 is to regulate neuronal differentiation, and that this function is mediated partly via its downstream IAP gene. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：199 / 206

页数：8

共 24 条

[1]

Becker TS, 1998, DEVELOPMENT, V125, P4369

[2] Integrin-associated protein (CD47) and its ligands [J].