Site-specific recombination for genetic engineering in plants

被引:44
作者
Lyznik, LA [1 ]
Gordon-Kamm, WJ [1 ]
Tao, Y [1 ]
机构
[1] Pioneer HiBred Int Inc, Transformat Res, Johnston, IA 50131 USA
关键词
site-specific recombination; plant transformation; genetic engineering; FLP/FRT; Cre/loxP;
D O I
10.1007/s00299-003-0616-7
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Site-specific recombination has been developed into a genetic engineering tool for higher eukaryotes. The manipulation of newly introduced DNA is now possible in the course of genetic transformation procedures, thus making the process more predictable and reliable. Also, a wide variety of chromosomal rearrangements using site-specific recombination have been documented both in metazoan and plant species. Applying such methods to plants opens new avenues for large-scale chromosome engineering in the future.
引用
收藏
页码:925 / 932
页数:8
相关论文
共 69 条
[1]   SITE-SPECIFIC INTEGRATION OF DNA INTO WILD-TYPE AND MUTANT LOX SITES PLACED IN THE PLANT GENOME [J].
ALBERT, H ;
DALE, EC ;
LEE, E ;
OW, DW .
PLANT JOURNAL, 1995, 7 (04) :649-659
[2]   Targeted integration of DNA using mutant lox sites in embryonic stem cells [J].
Araki, K ;
Araki, M ;
Yamamura, KI .
NUCLEIC ACIDS RESEARCH, 1997, 25 (04) :868-872
[3]   Coping with kinetic and thermodynamic barriers: RMCE, an efficient strategy for the targeted integration of transgenes [J].
Baer, A ;
Bode, J .
CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (05) :473-480
[4]  
Baszczynski CL, 2003, TRANSGENIC PLANTS: CURRENT INNOVATIONS AND FUTURE TRENDS, P157
[5]   Segmental genomic replacement by Cre-mediated recombination: Genotoxic stress activation of the p53 promoter in single-copy transformants [J].
Bethke, B ;
Sauer, B .
NUCLEIC ACIDS RESEARCH, 1997, 25 (14) :2828-2834
[6]   The transgeneticist's toolbox:: Novel methods for the targeted modification of eukaryotic genomes [J].
Bode, J ;
Schlake, T ;
Iber, M ;
Schübeler, D ;
Seibler, J ;
Snezhkov, E ;
Nikolaev, L .
BIOLOGICAL CHEMISTRY, 2000, 381 (9-10) :801-813
[7]   Alteration of Cre recombinase site specificity by substrate-linked protein evolution [J].
Buchholz, F ;
Stewart, AF .
NATURE BIOTECHNOLOGY, 2001, 19 (11) :1047-1052
[8]   Improved properties of FLP recombinase evolved by cycling mutagenesis [J].
Buchholz, F ;
Angrand, PO ;
Stewart, AF .
NATURE BIOTECHNOLOGY, 1998, 16 (07) :657-662
[9]  
Choi S, 2000, Nucleic Acids Res, V28, pE19, DOI 10.1093/nar/28.7.e19
[10]   Efficient elimination of selectable marker genes from the plastid genome by the CRE-lox site-specific recombination system [J].
Corneille, S ;
Lutz, K ;
Svab, Z ;
Maliga, P .
PLANT JOURNAL, 2001, 27 (02) :171-178