Evaluation of on-line viable biomass measurements during fermentations of Candida utilis

被引：19

作者：

November, EJ ^{[1
]}

Van Impe, JF ^{[1
]}

机构：

[1] Katholieke Univ Leuven, Dept Food & Microbial Technol, B-3001 Heverlee, Belgium

来源：

BIOPROCESS ENGINEERING | 2000年 / 23卷 / 05期

关键词：

Biomass; Fermentation; Candida; Specific Growth Rate; Fermentation Condition;

D O I：

10.1007/s004499900179

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this article the suitability of the Biomass Monitor for on-line measurement of viable biomass is thoroughly evaluated during aerobic fermentations of Candida utilis. Successively a number of specifications of the measuring device are discussed for the studied biological system. The optimal measurement frequency for the given experimental conditions is determined. Furthermore, reliable calibrations of the capacitance readings versus well-known off-line analysis of dry weight and plate counts of the yeast have been established. In addition, the impact of varying fermentation conditions such as stirrer speed and air flow rate together with the influence of the oxygen concentration and conductance of the medium on the capacitance signal have been studied and quantified when a significant influence was observed. It is illustrated that knowledge of the viable biomass during fermentations is very useful in the estimation of the specific growth rate of the organism.

引用

页码：473 / 477

页数：5