Hybrid bioelectrocatalyst for hydrogen peroxide reduction: Immobilization of enzyme within organic-inorganic film of structured Prussian Blue and PEDOT

被引：41

作者：

Ernst, Andrzej ^{[1
]}

Makowski, Oktawian ^{[1
]}

Kowalewska, Barbara ^{[1
]}

Miecznikowski, Krzysztof ^{[1
]}

Kulesza, Pawel J. ^{[1
]}

机构：

[1] Univ Warsaw, Dept Chem, PL-02093 Warsaw, Poland

来源：

BIOELECTROCHEMISTRY | 2007年 / 71卷 / 01期

关键词：

composite film; structured Prussian Blue film; 4(pyrrole-1-yl)-benzoic acid; poly(3,4-ethylendioxythiophene); horseradish peroxidase; bioelectrocatalysis;

D O I：

10.1016/j.bioelechem.2006.12.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fabrication of structured film (on glassy carbon substrate) composed of compact Prussian Blue (that has been prepared by alternate immersions and through assembling within ultra-thin layers of 4(pyrrole-1-yl)-benzoic acid, PPyBA) and poly(3,4-ethylendioxythiophene), PEDOT, is described. This functionalized film has been characterized by fast charge propagation, and it has served as a redox conducting template for permanent attachment of a model enzyme, horseradish peroxidase, HRP. The resulting organic-inorganic system acts as an effective hybrid bioelectrocatalyst for electroreduction of hydrogen peroxide, a model reactant for biosensors and biofuel cells. Among important issues are rigidity, permanence of enzyme attachment, morphology, hydrophilicity, and attractive mediating capabilities of the PEDOT-stabilized Prussian Blue based structured film. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：23 / 28

页数：6

共 39 条

[1] Effective charge transport in poly(3,4-ethylenedioxythiophene) based hybrid films containing polyoxometallate redox centers [J].