Layer-by-layer arrangement by protein-protein interaction of sulfite oxidase and cytochrome c catalyzing oxidation of sulfite

被引：76

作者：

Dronov, Roman ^{[2
,3
]}

Kurth, Dirk G. ^{[2
,4
]}

Moehwald, Helmuth ^{[2
]}

Spricigo, Roberto ^{[2
,3
]}

Leimkuehler, Silke ^{[3
]}

Wollenberger, Ulla ^{[3
]}

Rajagopalan, K. V. ^{[5
]}

Scheller, Frieder W. ^{[3
]}

Lisdat, Fred ^{[1
]}

机构：

[1] Wildau Univ Appl Sci, D-15745 Wildau, Germany

[2] Max Planck Inst Colloids & Interfaces, D-14424 Golm, Germany

[3] Univ Potsdam, Inst Biochem & Biol, D-14476 Golm, Germany

[4] Natl Inst Mat Sci, Tsukuba, Ibaraki 3050047, Japan

[5] Duke Univ, Med Ctr, Dept Biochem, Durham, NC 27710 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 04期

关键词：

D O I：

10.1021/ja0768690

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Layer-by-layer self-assembly of sulfite oxidase and cytochrome c was carried out without additional polymeric polyelectrolytes. The arrangement shows a linear increase in the immobilized protein mass after each deposition cycle. The modified electrodes demonstrate electrocatalytic activity for sulfite oxidation generating catalytic current with a linear increase with the number of layers. This effect shows that, in the protein assembly without a polyelectrolyte, electron transfer occurs, thus supporting the concept for direct interprotein electron exchange.

引用

页码：1122 / +

页数：3

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