Gibberellin dose-response regulation of GA4 gene transcript levels in Arabidopsis

被引:158
作者
Cowling, RJ
Kamiya, Y
Seto, H
Harberd, NP
机构
[1] John Innes Ctr, Dept Mol Genet, Norwich NR4 7UJ, Norfolk, England
[2] RIKEN, Frontier Res Program, Lab Plant Hormone Funct, Wako, Saitama 35101, Japan
[3] RIKEN, Plant Funct Lab, Wako, Saitama 35101, Japan
[4] RIKEN, Inst Phys & Chem Res, Wako, Saitama 35101, Japan
基金
英国生物技术与生命科学研究理事会;
关键词
D O I
10.1104/pp.117.4.1195
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The gibberellins (GAs) are a complex family of diterpenoid compounds, some of which are potent endogenous regulators of plant growth. As part of a feedback control of endogenous CA levels, active GAs negatively regulate the abundance of mRNA transcripts encoding CA biosynthesis enzymes. For example, Arabidopsis GA4 gene transcripts encode CA 3 beta-hydroxylase, an enzyme that catalyzes the conversion of inactive to active GAs. Here eve show that active GAs regulate GA4 transcript abundance in a dose-dependent manner, and that down-regulation of GA4 transcript abundance is effected by GA(4) (the product of SP-hydroxylation) but not by its immediate precursor GA(9) (the substrate). Comparison of several different CA structures showed that GAs active in promoting hypocotyl elongation were also active in regulating GA4 transcript abundance, suggesting that similar CA:receptor and subsequent signal transduction processes control these two responses. It is interesting that these activities were not restricted to 3 beta-hydroxylated GAs, being also exhibited by structures that were not 3 beta-hydroxylated but that had another electronegative group at C-3. We also show that CA-mediated control of GA4 transcript abundance is disrupted in the CA-response mutants gal and spy-ii. These observations define a sensitive homeostatic mechanism whereby plants may regulate their endogenous GA levels.
引用
收藏
页码:1195 / 1203
页数:9
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