Biomarker-assisted diagnosis of ovarian, cervical and pulmonary small cell carcinomas: the role of TTF-1, WT-1 and HPV analysis

被引:41
作者
Carlson, J. W.
Nucci, M. R.
Brodsky, J.
Crum, C. P.
Hirsch, M. S.
机构
[1] Brigham & Womens Hosp, Dept Pathol, Boston, MA 02115 USA
[2] Brigham & Womens Hosp, Harvard Med Sch, Div Womens & Perinatal Pathol, Boston, MA 02115 USA
关键词
cervix; cKIT; HPV; hypercalcaemic; lung; OCT3/4; ovary; p16; small cell carcinoma; TTF-1; WT-1;
D O I
10.1111/j.1365-2559.2007.02790.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Aims: Small cell carcinoma of the ovary, hypercalcaemic-type (SCCOH) is morphologically similar to small cell carcinomas from other sites. The aims of this study were to (i) determine if a biomarker panel would distinguish small cell carcinomas of the ovary, cervix (SCCCx) and lung (SCCLu) and (ii) potentially determine the histogenesis of SCCOH. Methods and results: Nine ovarian small cell carcinomas (seven hypercalcaemic type; two pulmonary type), eight SCCCx and 22 SCCLu were immunostained for thyroid transcription factor (TTF)-1, WT-1, p16, cKIT and OCT3/4; a subset of cases were tested for human papillornavirus (HPV). WT-1 was diffusely positive in 6/7 SSCOH versus two of 33 other small cell carcin-omas (P <= 0.001). TTF-1 was diffusely positive in 20/22 SCCLu and 1/8 SCCCx, and negative in all SCCOH. p16 and cKIT demonstrated variable patterns of immunoreactivity in all cases. HPV was identified in 5/6 SCCCx; SCCOH and SCCLu were negative for HPV. Conclusions: Combined staining with WT-1 and TTF-1 will distinguish SCCOH from SCCLu and SCCCx with a sensitivity of 86% and specificity of 97%. HPV is specific for tumours of cervical origin, but p16 immunohistochemistry is not useful for this purpose. The presence of diffuse WT-1 supports a Mullerian origin for SCCOH, whereas the absence of cKIT and OCT3/4 argues against a germ cell origin.
引用
收藏
页码:305 / 312
页数:8
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