Mapping the in vivo distribution of herpes simplex virions

被引:48
作者
Schellingerhout, D
Bogdanov, A
Marecos, E
Spear, M
Breakefield, X
Weissleder, R
机构
[1] Massachusetts Gen Hosp, Ctr Mol Imaging Res, Dept Radiol, Charlestown, MA 02129 USA
[2] Massachusetts Gen Hosp, Mol Neurogenet Unit, Charlestown, MA 02129 USA
关键词
D O I
10.1089/hum.1998.9.11-1543
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We describe a method for labeling enveloped viral particles with a radiotracer, indium-lll, allowing labeled viruses to be traced in viveo by nuclear imaging. After initial optimization experiments, a labeling efficiency of 83% (incorporation yield) was achieved for herpes simplex virus (HSV), resulting in a specific activity of 30 mu Ci/10(9) PFU, The labeling procedure did not significantly reduce the infectivity of the labeled virus and the virus did not release any significant amounts of the radionuclide within 12 hr after labeling. Sequential imaging of animals after intravenous administration of the labeled virus showed fast accumulation in the liver and redistribution from the blood pool (immediately after injection) to liver and spleen (12-24 hr after injection). At 12 hr after injection 7% of the virus-associated In-111 had been eliminated from the body and the remaining organ distribution of the virus was as follows: spleen 28.7 +/- 5.4% ID/g; liver, 26.0 +/- 5.1% ID/g; kidney, 9.8 +/- 3.1% ID/g; lung, 5.7 +/- 1.0% ID/g; and lower amounts in other organs, Our results indicate that the described method allows qualitative and quantitative assessment of viral biodistribution in vivo by nuclear imaging.
引用
收藏
页码:1543 / 1549
页数:7
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