Real-time luminescence reporting of circadian gene expression in mammals

被引:149
作者
Yamazaki, S
Takahashi, JS
机构
[1] Department of Biological Sciences, Vanderbilt University, Nashville
[2] Howard Hughes Medical Institute, Dept. of Neurobiology and Physiology, Northwestern University, Evanston
来源
CIRCADIAN RHYTHMS | 2005年 / 393卷
关键词
D O I
10.1016/S0076-6879(05)93012-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Luminescence reporters have been used successfully in studies of circadian rhythms. Real-time measurements of circadian variations in gene expression were made in living cells, cultured tissues, and whole organisms. Because this technique is relatively easy and continuous noninvasive measurement from tissue cultures allows for a drastic reduction in the number of experimental animals, we believe this method will become a common technique for studying circadian rhythms. Using a multichannel recording apparatus, it may also become a powerful tool for the discovery of new drugs. In the past, measurements were done using hand-made apparatuses or by modifying commercially available equipment. We, along with other investigators, have developed user-friendly equipment for performing circadian rhythms experiments, and these systems are now available commercially. This article describes the use of luminescence reporters in circadian research and provides detailed methods used in these experiments. One of our goals in this article is to reduce experimental variability in different laboratories by proposing standard protocols.
引用
收藏
页码:288 / 301
页数:14
相关论文
共 25 条
[1]  
[Anonymous], 2001, NEURON, DOI DOI 10.1016/S0896-6273(01)00302-6
[2]   Visualization of mPer1 transcription in vitro:: NMDA induces a rapid phase shift of mPer1 gene in cultured SCN [J].
Asai, M ;
Yamaguchi, S ;
Isejima, H ;
Jonouchi, M ;
Moriya, T ;
Shibata, S ;
Kobayashi, M ;
Okamura, H .
CURRENT BIOLOGY, 2001, 11 (19) :1524-1527
[3]   Multiple signaling pathways elicit circadian gene expression in cultured Rat-1 fibroblasts [J].
Balsalobre, A ;
Marcacci, L ;
Schibler, U .
CURRENT BIOLOGY, 2000, 10 (20) :1291-1294
[4]   Novel features of Drosophila period transcription revealed by real-time luciferase reporting [J].
Brandes, C ;
Plautz, JD ;
Stanewsky, R ;
Jamison, CF ;
Straume, M ;
Wood, KV ;
Kay, SA ;
Hall, JC .
NEURON, 1996, 16 (04) :687-692
[5]  
COLLACO AM, 2003, BMC PHYSL, V19, P8
[6]   Long-term monitoring of circadian rhythms in c-fos gene expression from suprachiasmatic nucleus cultures [J].
Geusz, ME ;
Fletcher, C ;
Block, GD ;
Straume, M ;
Copeland, NG ;
Jenkins, NA ;
Kay, SA ;
Day, RN .
CURRENT BIOLOGY, 1997, 7 (10) :758-766
[7]  
Geusz ME., 2001, Methods in Cellular Imaging. Basics of Fluorescence, Fluorophores, P395
[8]   Temporal precision in the mammalian circadian system: A reliable clock from less reliable neurons [J].
Herzog, ED ;
Aton, SJ ;
Numano, R ;
Sakaki, Y ;
Tei, H .
JOURNAL OF BIOLOGICAL RHYTHMS, 2004, 19 (01) :35-46
[9]   Circadian gene expression in mammalian fibroblasts revealed by real-time luminescence reporting: Temperature compensation and damping [J].
Izumo, M ;
Johnson, CH ;
Yamazaki, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) :16089-16094
[10]   CIRCADIAN-RHYTHMS IN PROKARYOTES - LUCIFERASE AS A REPORTER OF CIRCADIAN GENE-EXPRESSION IN CYANOBACTERIA [J].
KONDO, T ;
STRAYER, CA ;
KULKARNI, RD ;
TAYLOR, W ;
ISHIURA, M ;
GOLDEN, SS ;
JOHNSON, CH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5672-5676