Uptake and biological effects of chitosan-capped gold nanoparticles on Chinese Hamster Ovary cells

被引：55

作者：

Boca, Sanda C. ^{[1
]}

Potara, Monica ^{[1
]}

Toderas, Felicia ^{[1
]}

Stephan, Olivier ^{[2
]}

Baldeck, Patrice L. ^{[2
]}

Astilean, Simion ^{[1
]}

机构：

[1] Univ Babes Bolyai, Nanobiophoton Ctr, Inst Interdisciplinary Expt Res, Cluj Napoca 400271, Romania

[2] Univ Grenoble 1, Lab Spectrometrie Phys, CNRS, UMR 5588, F-38402 St Martin Dheres, France

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2011年 / 31卷 / 02期

关键词：

Gold nanoparticles; Chitosan; Cell viability; DRUG-DELIVERY; IN-VITRO; SHAPE; SIZE; ABSORPTION; NANORODS; TUMOR;

D O I：

10.1016/j.msec.2010.08.015

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

In this study we examine the cytotoxic effect of chitosan capped-gold nanoparticles on Chinese Hamster Ovary (CHO) cells in vitro. The particles were synthesized through a low cost and rapid method by the aqueous reduction of chloroauric acid (HAuCl4) with ascorbic acid (C6H8O6) at ambient temperature. High molecular weight chitosan biopolymer was used to stabilize the particles against aggregation. The conjugated particles were able to traverse the cell membrane and enter the cells by endocytic pathway, their intracellular presence being clearly revealed by dark field microscopy imaging and light scattering spectra. Gold nanoparticles cytotoxicity was measured and cells were found to be viable more than 85%, even after long time exposure. Our results suggest that chitosan-conjugated gold nanoparticles have minimal impact on cells viability and morphology during 19 h demonstrating great potential to be used in biomedical applications such as cellular imaging or photothermal therapy. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：184 / 189

页数：6

共 42 条

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