Multilocus Sequence Typing of Pseudomonas syringae Sensu Lato Confirms Previously Described Genomospecies and Permits Rapid Identification of P. syringae pv. coriandricola and P. syringae pv. apii Causing Bacterial Leaf Spot on Parsley

被引:90
作者
Bull, Carolee T. [1 ]
Clarke, Christopher R. [2 ]
Cai, Rongman [2 ]
Vinatzer, Boris A. [2 ]
Jardini, Teresa M. [1 ]
Koike, Steven T. [3 ]
机构
[1] ARS, USDA, Salinas, CA 93905 USA
[2] Virginia Tech, Plant Pathol Physiol & Weed Sci Dept, Blacksburg, VA 24061 USA
[3] Univ Calif Cooperat Extens, Salinas, CA 93901 USA
基金
美国国家科学基金会;
关键词
Apiaceae; Apium graveolens; Coriandrum sativum; DNA/DNA hybridization; species concept; RIBOSOMAL-RNA GENE; AD-HOC-COMMITTEE; PATHOVARS; CILANTRO; NOV; AMPLIFICATION; RELATEDNESS; EVOLUTION; CORIANDER; DISEASES;
D O I
10.1094/PHYTO-11-10-0318
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Bull, C. T., Clarke, C. R., Cai, R., Vinatzer, B. A., Jardini, T. M., and Koike, S. T. 2011. Multi locus sequence typing of Pseudomonas syringae sensu lato confirms previously described genomospecies and permits rapid identification of P syringae pv. coriandricola and P. syringae pv. apii causing bacterial leaf spot on parsley. Phytopathology 101:847-858. Since 2002, severe leaf spotting on parsley (Petroselinum crispum) has occurred in Monterey County, CA. Either of two different pathovars Of Pseudomonas syringae sensu lato were isolated from diseased leaves from eight distinct outbreaks and once from the same outbreak. Fragment analysis of DNA amplified between repetitive sequence polymerase chain reaction; 16S rDNA sequence analysis; and biochemical, physiological, and host range tests identified the pathogens as Pseudomonas syringae pv. apii and P. syringae pv. coriandricola. Koch's postulates were completed for the isolates from parsley, and host range tests with parsley isolates and pathotype strains demonstrated that P syringae pv. apii and P syringae pv. coriandricola cause leaf spot diseases on parsley, celery, and coriander or cilantro. In a multilocus sequence typing (MLST) approach, four housekeeping gene fragments were sequenced from 10 strains isolated from parsley and 56 pathotype strains of P. syringae. Allele sequences were uploaded to the Plant-Associated Microbes Database and a phylogenetic tree was built based on concatenated sequences. Tree topology directly corresponded to P. syringae genomospecies and P. syringae pv. apii was allocated appropriately to genomospecies 3. This is the first demonstration that MLST can accurately allocate new pathogens directly to P. syringae sensu lato genomospecies. According to MLST, P syringae pv. coriandricola is a member of genomospecies 9, P. cannabina. In a blind test, both P syringae pv. coriandricola and P. syringae pv. apii isolates from parsley were correctly identified to pathovar. In both cases, MLST described diversity within each pathovar that was previously unknown.
引用
收藏
页码:847 / 858
页数:12
相关论文
共 66 条
[1]   PAMDB, A Multilocus Sequence Typing and Analysis Database and Website for Plant-Associated Microbes [J].
Almeida, Nalvo F. ;
Yan, Shuangchun ;
Cai, Rongman ;
Clarke, Christopher R. ;
Morris, Cindy E. ;
Schaad, Norman W. ;
Schuenzel, Erin L. ;
Lacy, George H. ;
Sun, Xiaoan ;
Jones, Jeffrey B. ;
Castillo, Jose A. ;
Bull, Carolee T. ;
Leman, Scotland ;
Guttman, David S. ;
Setubal, Joao C. ;
Vinatzer, Boris A. .
PHYTOPATHOLOGY, 2010, 100 (03) :208-215
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence [J].
Anzai, Y ;
Kim, H ;
Park, JY ;
Wakabayashi, H ;
Oyaizu, H .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2000, 50 :1563-1589
[4]   IDENTIFICATION AND RELATEDNESS OF CORONATINE-PRODUCING PSEUDOMONAS-SYRINGAE PATHOVARS BY PCR ANALYSIS AND SEQUENCE DETERMINATION OF THE AMPLIFICATION PRODUCTS [J].
BERESWILL, S ;
BUGERT, P ;
VOLKSCH, B ;
ULLRICH, M ;
BENDER, CL ;
GEIDER, K .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (08) :2924-2930
[5]  
BOCK C, 2005, PHYTOPATHOLOGY S, V95, pS12
[6]  
BOCK C, 2005, PHYTOPATHOLOGY S, V95, pS11
[7]  
Bradbury J.F., 1986, GUIDE PLANT PATHOGEN
[8]   Pantoea vagans sp nov., Pantoea eucalypti sp nov., Pantoea deleyi sp nov and Pantoea anthophila sp nov. [J].
Brady, Carrie L. ;
Venter, Stephanus N. ;
Cleenwerck, Ilse ;
Engelbeen, Katrien ;
Vancanneyt, Marc ;
Swings, Jean ;
Coutinho, Teresa A. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2009, 59 :2339-2345
[9]  
BROSIUS J, 1978, P NATL ACAD SCI USA, V75, P4801, DOI 10.1073/pnas.75.10.4801
[10]  
Bull CT, 2010, J PLANT PATHOL, V92, P551