YjgF is required for isoleucine biosynthesis when Salmonella enterica is grown on pyruvate medium

被引：41

作者：

Christopherson, Melissa R. ^{[1
]}

Schmitz, G. E. ^{[1
]}

Downs, Diana M. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Bacteriol, Madison, WI 53706 USA

来源：

JOURNAL OF BACTERIOLOGY | 2008年 / 190卷 / 08期

关键词：

D O I：

10.1128/JB.01700-07

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The YjgF/YER057c/UK114 family of proteins is conserved across the three domains of life, yet no biochemical function has been clearly defined for any member of this family. In Salmonella enterica, a deletion of yjgF results in a requirement for isoleucine when the mutant strain is grown in glucose-serine or pyruvate medium. Feedback inhibition of IlvA is required for the curative effect of isoleucine on glucose-serine medium. On pyruvate medium, yjgF mutants are unable to synthesize enough isoleucine for growth. From this study, we conclude that the isoleucine requirement of a yjgF mutant on pyruvate is a consequence of the decreased transaminase B (IlvE) activity that has previously been characterized in these mutants.

引用

页码：3057 / 3062

页数：6

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