Activation of mouse phosphodiesterase 3B gene promoter by adipocyte differentiation in 3T3-L1 cells

被引：18

作者：

Niiya, T

Osawa, H ^{[1
]}

Onuma, H

Suzuki, Y

Taira, M

Yamada, K

Makino, H

机构：

[1] Ehime Univ, Sch Med, Dept Lab Med, Shigenobu, Ehime 7910295, Japan

[2] Chiba Univ, Sch Med, Dept 2, Chiba 280, Japan

[3] Fukui Med Univ, Dept Biochem, Fukui, Japan

[4] Japan Sci & Technol, CREST, Fukui, Japan

来源：

FEBS LETTERS | 2001年 / 505卷 / 01期

基金：

日本学术振兴会;

关键词：

phosphodiesterase; 3B; adipocyte; differentiation; transcription; promoter; insulin resistance;

D O I：

10.1016/S0014-5793(01)02807-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Activation of phosphodiesterase (PDE) 3B reduces free fatty acid output from adipocytes. Induction of PDE3B gene expression by adipocyte differentiation could improve insulin resistance. To examine whether the PDE3B promoter is activated by this differentiation, the 5 ' flanking sequence of the mouse PDE3B gene was isolated. The transcription initiation site was determined to be located 195 bp upstream of the translation start site. No putative binding site for peroxisome proliferator-activated receptor gamma was, found within 2 kb upstream of the transcription initiation site. This region had promoter activity, which was, further activated on adipocyte differentiation in 3T3-L1 cells. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：136 / 140

页数：5

共 25 条

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