Imaging of Phospholipids in Formalin Fixed Rat Brain Sections by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry

被引：67

作者：

Carter, Claire L. ^{[1
]}

McLeod, Cameron W. ^{[2
]}

Bunch, Josephine ^{[1
]}

机构：

[1] Univ Birmingham, Sch Chem, Birmingham B15 2TT, W Midlands, England

[2] Univ Sheffield, Dept Chem, Ctr Analyt Sci, Sheffield S3 7HF, S Yorkshire, England

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2011年 / 22卷 / 11期

基金：

英国工程与自然科学研究理事会;

关键词：

MALDI-MS; Mass spectrometry imaging; Phospholipids; Formalin fixed tissue; Ionisation pathways; MALDI-TOF-MS; MAMMALIAN TISSUE-SECTIONS; ION MOBILITY-TOFMS; MOUSE-BRAIN; PROTEIN EXPRESSION; SMALL MOLECULES; FABRY-DISEASE; LIPIDS; IMMUNOHISTOCHEMISTRY; SPHINGOLIPIDS;

D O I：

10.1007/s13361-011-0227-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a valuable tool for the analysis of molecules directly from tissue. Imaging of phospholipids is gaining widespread interest, particularly as these lipids have been implicated in a variety of pathologic processes. Formalin fixation (FF) is the standard protocol used in histology laboratories worldwide to preserve tissue for analysis, in order to aid in the diagnosis and prognosis of diseases. This study assesses MALDI imaging of phospholipids directly in formalin fixed tissue, with a view to future analysis of archival tissue. This investigation proves the viability of MALDI-MSI for studying the distribution of lipids directly in formalin fixed tissue, without any pretreatment protocols. High quality molecular images for several phosphatidylcholine (PC) and sphingomyelin (SM) species are presented. Images correspond well with previously published data for the analysis of lipids directly from freshly prepared tissue. Different ionization pathways are observed when analyzing fixed tissue compared with fresh, and this change was found to be associated with formalin buffers employed in fixation protocols. The ability to analyze lipids directly from formalin fixed tissue opens up new doors in the investigation of disease profiles. Pathologic specimens taken for histologic investigation can be analyzed by MALDI-MS to provide greater information on the involvement of lipids in diseased tissue.

引用

页码：1991 / 1998

页数：8

共 76 条

[1]

ALLRED DC, 2005, SEMIN BREAST DIS, V8, P57

[2]

Aoki Y, 2007, P JPN ACAD B-PHYS, V83, P205, DOI [10.2183/pjab.83.205, 10.2183/pjab/83.205]

[3] Profiling and Imaging of Lipids on Brain and Liver Tissue by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using 2-Mercaptobenzothiazole as a Matrix [J].

Astigarraga, Egoitz ;

Barreda-Gomez, Gabriel ;

Lombardero, Laura ;

Fresnedo, Olatz ;

Castano, Fernando ;

Giralt, Maria Teresa ;

Ochoa, Begona ;

Rodriguez-Puertas, Rafael ;

Fernandez, Jose A. .

ANALYTICAL CHEMISTRY, 2008, 80 (23) :9105-9114

[4] Automated MALDI matrix deposition method with inkjet printing for imaging mass spectrometry [J].

Baluya, Dodge L. ;

Garrett, Timothy J. ;

Yost, Richard A. .

ANALYTICAL CHEMISTRY, 2007, 79 (17) :6862-6867

[5] MALDI reveals membrane lipid profile reversion in MDX mice [J].

Benabdellah, Farida ;

Yu, Hua ;

Brunelle, Alain ;

Laprevote, Olivier ;

De La Porte, Sabine .

NEUROBIOLOGY OF DISEASE, 2009, 36 (02) :252-258

[6] Histology without formalin? [J].

Buesa, Rene J. .

ANNALS OF DIAGNOSTIC PATHOLOGY, 2008, 12 (06) :387-396

[7] Determination of pharmaceutical compounds in skin by imaging matrix-assisted laser desorption/ionisation mass spectrometry [J].

Bunch, J ;

Clench, MR ;

Richards, DS .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2004, 18 (24) :3051-3060

[8] Spatial and temporal alterations of phospholipids determined by mass spectrometry during mouse embryo implantation [J].

Burnum, Kristin E. ;

Cornett, Dale S. ;

Puolitaival, Satu M. ;

Milne, Stephen B. ;

Myers, David S. ;

Tranguch, Susanne ;

Brown, H. Alex ;

Dey, Sudhansu K. ;

Caprioli, Richard M. .

JOURNAL OF LIPID RESEARCH, 2009, 50 (11) :2290-2298

[9] Molecular imaging of biological samples: Localization of peptides and proteins using MALDI-TOF MS [J].

Caprioli, RM ;

Farmer, TB ;

Gile, J .

ANALYTICAL CHEMISTRY, 1997, 69 (23) :4751-4760

[10] Direct profiling of myelinated and demyelinated regions in mouse brain by imaging mass spectrometry [J].

Ceuppens, Ruben ;

Dumont, Debora ;

Van Brussel, Leen ;

Van de Plas, Babs ;

Daniels, Ruth ;

Noben, Jean-Paul ;

Verhaert, Peter ;

Van der Gucht, Estel ;

Robben, Johan ;

Clerens, Stefan ;

Arckens, Lutgarde .

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2007, 260 (2-3) :185-194

← 1 2 3 4 5 6 7 8 →