Rapid detection of mutations associated with resistance to erythromycin in Campylobacter jejuni/coli by PCR and line probe assay

被引:31
作者
Niwa, H
Chuma, T
Okamoto, K
Itoh, K
机构
[1] Univ Tokyo, Grad Sch Agr & Life Sci, Dept Vet Publ Hlth, Bunkyo Ku, Tokyo 1138657, Japan
[2] Kagoshima Univ, Fac Agr, Dept Vet Med, Lab Vet Publ Hlth, Kagoshima 8900065, Japan
关键词
23S rRNA gene mutations; Campylobacter jejuni/coli; antibiotic resistance; macrolide; reverse hybridization;
D O I
10.1016/S0924-8579(01)00425-3
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Mutation of 23S rDNA is one of the mechanisms of erythromycin resistance. PCR and line probe assay (PCR-LiPA) with ten oligonucleotide probes were developed to detect the mutations associated with macrolide resistance at positions of 2072, 2073 and 2074 in 23S rDNA of Campylobacter jejuni/coli. A2074 --> G mutation was detected in 12 of 25 isolates, which were resistant to erythromycin. No other mutations in 23S rDNA were detected. The rest of the strains were susceptible to erythromycin and no mutation in 23S rDNA was detected. Six laboratory induced erythromycin resistant mutants had no mutations in 23S rDNA. PCR-LiPA is a useful and rapid method to detect mutations in 23S rDNA associated with erythromycin resistance in C. jejuni/coli. (C) 2001 Elsevier Science B.V. and International Society of Chemotherapy, All rights reserved.
引用
收藏
页码:359 / 364
页数:6
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