A non-isotopic method for the detection of telomerase activity in tumour tissues: TRAP-silver staining assay

被引：26

作者：

Wen, JM ^{[1
]}

Sun, LB

Zhang, M

Zheng, MH

机构：

[1] Sun Yat Sen Univ Med Sci, Dept Pathol, Guangzhou 510089, Peoples R China

[2] Univ Western Australia, Dept Orthopaed Surg, Nedlands, WA 6009, Australia

来源：

JOURNAL OF CLINICAL PATHOLOGY-MOLECULAR PATHOLOGY | 1998年 / 51卷 / 02期

关键词：

telomerase; TRAP; bone tumours; silver staining;

D O I：

10.1136/mp.51.2.110

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Telomerase activity has been observed in a high proportion of specimens from a wide variety of human malignant tumours, but not in non-neoplastic tissues. Measurement of telomerase activity is a possible diagnostic maker for malignant tumours. A non-isotopic telomeric repeat amplification protocol (TRAP) silver staining method for the detection of telomerase activity in human bone tumour tissues is described. The TRAP-silver staining method was quick, safe, and effective, and can be used as a routine diagnostic method for the detection of telomerase activity in bone tumours.

引用

页码：110 / 112

页数：3

共 14 条

[1] Telomerase activity in reactive and neoplastic lymphoid tissues: Infrequent detection of activity in Hodgkin's disease [J].

Brousset, P ;

AlSaati, T ;

Chaouche, N ;

Zenou, RC ;

Schlaifer, D ;

Chittal, S ;

Delsol, G .

BLOOD, 1997, 89 (01) :26-31

[2]

Califano J, 1996, CANCER RES, V56, P5720

[3]

CHADENEAU C, 1995, CANCER RES, V55, P2533

[4] TELOMERASE ACTIVITY IN HUMAN OVARIAN-CARCINOMA [J].

COUNTER, CM ;

HIRTE, HW ;

BACCHETTI, S ;

HARLEY, CB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (08) :2900-2904

[5] CORRELATING TELOMERASE ACTIVITY LEVELS WITH HUMAN NEUROBLASTOMA OUTCOMES [J].

HIYAMA, E ;

HIYAMA, K ;

YOKOYAMA, T ;

MATSUURA, Y ;

PIATYSZEK, MA ;

SHAY, JW .

NATURE MEDICINE, 1995, 1 (03) :249-255

[6] TELOMERASE ACTIVITY IN SMALL-CELL AND NON-SMALL-CELL LUNG CANCERS [J].

HIYAMA, K ;

HIYAMA, E ;

ISHIOKA, S ;

YAMAKIDO, M ;

INAI, K ;

GAZDAR, AF ;

PIATYSZEK, MA ;

SHAY, JW .

JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1995, 87 (12) :895-902

[7] SPECIFIC ASSOCIATION OF HUMAN TELOMERASE ACTIVITY WITH IMMORTAL CELLS AND CANCER [J].

KIM, NW ;

PIATYSZEK, MA ;

PROWSE, KR ;

HARLEY, CB ;

WEST, MD ;

HO, PLC ;

COVIELLO, GM ;

WRIGHT, WE ;

WEINRICH, SL ;

SHAY, JW .

SCIENCE, 1994, 266 (5193) :2011-2015

[8]

KOJMA H, 1997, GASTROENTEROLOGY, V112, P493

[9] Telomerase activity in primary prostate cancer [J].

Lin, Y ;

Uemura, H ;

Fujinami, K ;

Hosaka, M ;

Harada, M ;

Kubota, Y .

JOURNAL OF UROLOGY, 1997, 157 (03) :1161-1165

[10]

Sugino T, 1996, INT J CANCER, V69, P301, DOI 10.1002/(SICI)1097-0215(19960822)69:4<301::AID-IJC11>3.0.CO

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